腹裂胸动物肽YY的分子特征和组织表达:餐后变化和禁食对下丘脑表达的影响

Regulatory Peptides Pub Date : 2014-05-01 DOI:10.1016/j.regpep.2014.03.004

Dengyue Yuan, Chaowei Zhou, Tao Wang, Fangjun Lin, Hu Chen, Hongwei Wu, Rongbin Wei, Zhiming Xin, Ju Liu, Yundi Gao, Defang Chen, Shiyong Yang, Yan Wang, Yundan Pu, Zhiqiong Li

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引用次数: 24

摘要

肽YY (PYY)是一种有效的厌食神经肽，涉及哺乳动物的摄食调节。然而，PYY在硬骨鱼摄食行为中的作用尚未得到很好的理解。本研究采用分子、实时定量PCR和生理研究等方法，对南裂腹(Schizothorax prenanti, S. prenanti)中PYY的结构、分布和食欲调节作用进行了研究。在硬鱼中发现PYY序列具有很高的保守性。PYY表达广泛，在猪的端脑、延髓、垂体和下丘脑表达水平最高。下丘脑PYY mRNA的表达在餐后显著升高，提示PYY在大鼠中具有饱腹感信号作用。此外，PYY基因在下丘脑的表达在禁食后下降，再喂养后急剧上升，这表明PYY可能参与了虾蛄食欲的中枢调节。本研究结果为进一步研究红腹螺摄食规律提供了依据。

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Molecular characterization and tissue expression of peptide YY in Schizothorax prenanti: Effects of periprandial changes and fasting on expression in the hypothalamus

Peptide YY (PYY) is a potent anorectic neuropeptide implicated in feeding regulation in mammals. However, the involvement of PYY in the feeding behavior of teleosts has not been well understood. In this study, we employed molecular, real-time quantitative PCR and physiological studies to characterize the structure, distribution, and appetite regulatory effects of PYY in Schizothorax prenanti (S. prenanti). A very high conservation in PYY sequences was found in teleosts. PYY is widely expressed, with the highest levels of expression in telencephalon, medulla oblongata, pituitary and hypothalamus of S. prenanti. The PYY mRNA expression in the hypothalamus was highly elevated after a meal, suggesting a satiety signal role for PYY in S. prenanti. In addition, PYY gene expression in the hypothalamus was decreased after fasting and increased sharply after refeeding, which suggested that PYY might be involved in the central regulation of appetite in S. prenanti. Overall, our result provides basis for further investigation into the regulation of feeding in S. prenanti.

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

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审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.