回肠介入手术对UC Davis 2型糖尿病大鼠肠内分泌细胞数量的影响

Regulatory Peptides Pub Date : 2014-02-10 DOI:10.1016/j.regpep.2014.01.002

Carl Frederik Hansen , Efstathios Vassiliadis , Niels Vrang , Per T. Sangild , Bethany P. Cummings , Peter Havel , Jacob Jelsing

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引用次数: 23

摘要

目的探讨回肠介入手术(IT)对糖尿病前期UC Davis 2型糖尿病(UCD-T2DM)大鼠肠道形态和肠内分泌细胞数量的短期影响。研究设计两个月大的雄性UCD-T2DM大鼠分别接受假手术(n = 5)和IT手术(n = 5)。术后1.5个月收集肠道。对空肠、回肠和结肠区域进行组织化学和免疫组织化学标记，并对肠道形态和肠内分泌细胞数量的变化进行体视学分析。结果体视学分析显示，与假手术动物相比，IT大鼠的肠体积、肠管表面积和所有嗜铬粒蛋白a阳性肠内分泌细胞数量均明显增加。对IT区肠内分泌细胞的胰高血糖素样肽2、胆囊收缩素、血清素细胞和神经紧张素免疫反应亚池的亚分析显示，不同表型的数量都有所增加。然而，不同类型细胞的密度有所不同。结论UCD-T2DM大鼠行it手术后，肠道形态改变迅速，肠内分泌细胞数量增加。这一效应可能解释了为什么IT手术延迟了UCD-T2DM大鼠2型糖尿病的发病。

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The effect of ileal interposition surgery on enteroendocrine cell numbers in the UC Davis type 2 diabetes mellitus rat

Aim

To investigate the short-term effect of ileal interposition (IT) surgery on gut morphology and enteroendocrine cell numbers in the pre-diabetic UC Davis type 2 diabetes mellitus (UCD-T2DM) rat.

Study design

Two-month old male UCD-T2DM rats underwent either sham (n = 5) or IT (n = 5) surgery. Intestines were collected 1.5 months after surgery. The jejunum, ileum and colon regions were processed for histochemical and immunohistochemical labeling and stereological analyses of changes in gut morphometry and number of enteroendocrine cells.

Results

Stereological analysis showed that intestinal volume, luminal surface area and the number of all chromogranin A-positive enteroendocrine cells were markedly increased in the IT rats compared with sham-operated animals. Subanalyses of the glucagon-like peptide 2, cholecystokinin, serotonin cells and the neurotensin immunoreactive sub-pool of enteroendocrine cells in the IT region revealed an increase in numbers across phenotypes. However, the density of the different cell types varied.

Conclusion

IT surgery in the UCD-T2DM rat leads to rapid alterations in gut morphometry and an increase in the number of enteroendocrine cells. This effect may potentially explain why IT surgery delays the onset of type 2 diabetes in the UCD-T2DM rat.

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.