焦磷酸测序:强大的定量测序技术

Q2 Biochemistry, Genetics and Molecular Biology

Current Protocols in Molecular Biology Pub Date : 2018-02-16 DOI:10.1002/0471142727.mb0715s104

Martin Kreutz, Norbert Hochstein, Julia Kaiser, Frank Narz, Ralf Peist

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引用次数: 10

摘要

焦磷酸测序是一种用于DNA分析的合成测序方法，它不仅是从头测序应用的平台，也是基因组甲基化、单核苷酸多态性和等位基因定量分析的平台。在本单元中，我们描述了一个完整的工作流程，从样本到结果，是适合于每个这些应用程序。由于胞嘧啶转化是使用焦磷酸测序成功进行甲基化分析的关键因素，因此还包括亚硫酸氢盐处理的支持方案。咕咕叫。Protoc。摩尔。生物104:7.15.1-7.15.23。©2013 by John Wiley &儿子,Inc。

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Pyrosequencing: Powerful and Quantitative Sequencing Technology

Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification. In this unit, we describe a complete workflow from sample to result that is suitable for each of these applications. As cytosine conversion is a key element of successful methylation analysis using pyrosequencing, a support protocol for bisulfite treatment is also included. Curr. Protoc. Mol. Biol. 104:7.15.1-7.15.23. © 2013 by John Wiley & Sons, Inc.

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Current Protocols in Molecular Biology Biochemistry, Genetics and Molecular Biology-Molecular Biology

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