人诺如病毒的检测及病毒样颗粒的生产、定量和储存

Current Protocols in Microbiology Pub Date : 2018-02-16 DOI:10.1002/9780471729259.mc15k01s31

Kari Debbink, Veronica Costantini, Jesica Swanstrom, Sudhakar Agnihothram, Jan Vinjé, Ralph Baric, Lisa Lindesmith

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引用次数: 30

摘要

人类诺如病毒在世界范围内构成重大疾病负担。每年，诺如病毒导致超过2.67亿人感染，20多万5岁以下儿童死亡，以及超过50%的美国食源性疾病。由于缺乏组织培养模型或小动物模型来研究人类诺如病毒，病毒样颗粒(vlp)和基于elisa的生物测定已被用于回答有关诺如病毒进化和免疫的问题，并提供潜在的疫苗平台。本章概述了粪便中诺如病毒检测的方案，以及使用委内瑞拉马脑炎病毒(VEE)为基础的病毒复制子颗粒(VRP)表达系统设计、生产、纯化和储存诺如病毒VLP。咕咕叫。Protoc。Microbiol 31:15K.1.1-15K.1.45。©2013 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Human Norovirus Detection and Production, Quantification, and Storage of Virus-Like Particles

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Human Norovirus Detection and Production, Quantification, and Storage of Virus-Like Particles

Human noroviruses constitute a significant worldwide disease burden. Each year, noroviruses cause over 267 million infections, deaths in over 200,000 children under the age of five, and over 50% of U.S. food-borne illness. Due to the absence of a tissue culture model or small animal model to study human norovirus, virus-like particles (VLPs) and ELISA-based biological assays have been used to answer questions about norovirus evolution and immunity as well to provide a potential vaccine platform. This chapter outlines the protocols for norovirus detection in stool, as well as norovirus VLP design, production, purification, and storage using a Venezuelan equine encephalitis virus (VEE)–based virus replicon particle (VRP) expression system. Curr. Protoc. Microbiol. 31:15K.1.1-15K.1.45. © 2013 by John Wiley & Sons, Inc.

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Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.