利用线粒体基因PCR-RFLP鉴定4株中华鳖。

Mitochondrial Dna Pub Date : 2015-08-01 Epub Date: 2014-01-17 DOI:10.3109/19401736.2013.869674

Chao Zhang, Xiao-Jun Xu, Hai-Qi Zhang, Chang-Kao Mu, Zhong-Yang He, Chun-Lin Wang

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引用次数: 6

摘要

建立了一种PCR-RFLP方法，对4株中华白颡鱼(太湖株、台湾株、黄河株和日本株)进行鉴定。在这项研究中，我们对来自4个中华按蚊菌株的60个个体的线粒体NADH4、COX I和NADH5-NADH6基因的部分序列进行了测序和分析。选择Bgl I、Hpa II和Cla I分别切割NADH4、COX I和NADH5-NADH6 PCR产物，每种菌株都有其独特的限制性内切带模式。结果表明，根据三种酶解片段模式的组合，140个样品均能正确鉴定。本研究提供了一种有效的方法来区分中华p.p sinensis的四种主要菌株。

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PCR-RFLP identification of four Chinese soft-shelled turtle Pelodiscus sinensis strains using mitochondrial genes.

A PCR-RFLP method to confirm the identification of four Pelodiscus sinensis strains (Taihu Lake strain, Taiwan strain, Yellow River strain and Japanese strain) was developed and evaluated. In this study, we sequenced and analyzed the partial sequences of mitochondrial NADH4, COX I and NADH5-NADH6 genes of 60 individuals from the four P. sinensis strains. Bgl I, Hpa II and Cla I were selected for cutting NADH4, COX I and NADH5-NADH6 PCR products, respectively, and each strain has its unique restriction band patterns. The result showed that all the 140 samples tested can be correctly identified based on the combination of the three digested fragments pattern. This study provides an effective method to distinguish the four main strains of P. sinensis.

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来源期刊

Mitochondrial Dna 生物-遗传学

自引率

0.00%

发文量

审稿时长

2.4 months

期刊介绍： Previously published under the title DNA Sequence (Vols 1-19.3), Mitochondrial DNA accepts original high-quality reports based on mapping, sequencing and analysis of mitochondrial DNA and RNA. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, medical genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The editorial board will also consider manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences.