线粒体DNA编码区核苷酸位置10,716-11,184的单倍型和可变位置检测。

Mitochondrial Dna Pub Date : 2015-08-01 Epub Date: 2014-01-07 DOI:10.3109/19401736.2013.869675

Imad Hadi Hameed, Ameer Ibrahim Abdulzahra, Mohammed Abdullah Jebor, Cheah Yoke Kqueen, Aamera Jaber Ommer

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引用次数: 31

摘要

本研究利用Sanger测序法对线粒体非编码区进行鉴定，以供法医学应用。采用FTA®技术(FTA™纸DNA提取)提取DNA。编码区(10,716 ~ 11,184)部分根据Anderson参考序列扩增。用EZ-10自旋柱纯化的PCR产物，用ABI 3730 × L DNA分析仪进行测序和检测。所描述的新的多态性位点10,750和10,790可能是将来用于鉴定目的的合适来源。获得的数据可用于识别以频繁出现为特征的可变核苷酸位置，最有希望用于识别变异。

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Haplotypes and variable position detection in the mitochondrial DNA coding region encompassing nucleotide positions 10,716-11,184.

This study evaluates the mitochondrial noncoding regions by using the Sanger sequencing method for application in Forensic Science. FTA® Technology (FTA™ paper DNA extraction) was utilized to extract DNA. Portion of coding region encompassing positions from (10,716 to 11,184) amplified in accordance with the Anderson reference sequence. PCR products purified by EZ-10 spin column were then sequenced and detected using the ABI 3730 × L DNA Analyzer. A new polymorphic positions 10,750 and 10,790 that are described may be suitable sources in future for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence, most promising for identification variants.

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来源期刊

Mitochondrial Dna 生物-遗传学

自引率

0.00%

发文量

审稿时长

2.4 months

期刊介绍： Previously published under the title DNA Sequence (Vols 1-19.3), Mitochondrial DNA accepts original high-quality reports based on mapping, sequencing and analysis of mitochondrial DNA and RNA. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, medical genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The editorial board will also consider manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences.