{"title":"[2008 - 2009年北京市G II.12诺如病毒遗传特征分析]。","authors":"Ke-Na Chen, Geng Tian, Miao Jin, Hui-Ying Li, Quan-Rui Li, Li-Hong Kang, Cui-Hong Zhang, Xiang-Yu Kong, Ji-In Gao, Zhao-Jun Duan","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To reveal the genetic characteristics of GII.12 Norovirus strains isolating from stool samples of adults with diarrhea in Beijing during 2008-2009.</p><p><strong>Methods: </strong>RdRp, ORF2, ORF3 and ORF1/ORF2 overlap region were respectively amplified by primers using RT-PCR. The products were purified, cloned, sequenced and then aligned, phylogenetic and recombinant analyzed by softwares of DNAStar, MEGA and SimPlot.</p><p><strong>Results: </strong>According to the phylogenetic analysis, 11 strains belonged to G II.g in the RdRp region,while GII.12 in the ORF2 and ORF3. SimPlot analysis further confirmed the 11 strains were recombinant strains ( G II.g [RdRp]/G II.12 [capsid]).</p><p><strong>Conclusion: </strong>G II.12 Norovirus prevailing in Beijing and other regions of the world belonged to the same strain, and we identified the genetic characteristics of G II.12 Norovirus in Beijing.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 3","pages":"184-6"},"PeriodicalIF":0.0000,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Genetic characterization of G II.12 norovirus in Beijing from 2008 to 2009].\",\"authors\":\"Ke-Na Chen, Geng Tian, Miao Jin, Hui-Ying Li, Quan-Rui Li, Li-Hong Kang, Cui-Hong Zhang, Xiang-Yu Kong, Ji-In Gao, Zhao-Jun Duan\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To reveal the genetic characteristics of GII.12 Norovirus strains isolating from stool samples of adults with diarrhea in Beijing during 2008-2009.</p><p><strong>Methods: </strong>RdRp, ORF2, ORF3 and ORF1/ORF2 overlap region were respectively amplified by primers using RT-PCR. The products were purified, cloned, sequenced and then aligned, phylogenetic and recombinant analyzed by softwares of DNAStar, MEGA and SimPlot.</p><p><strong>Results: </strong>According to the phylogenetic analysis, 11 strains belonged to G II.g in the RdRp region,while GII.12 in the ORF2 and ORF3. SimPlot analysis further confirmed the 11 strains were recombinant strains ( G II.g [RdRp]/G II.12 [capsid]).</p><p><strong>Conclusion: </strong>G II.12 Norovirus prevailing in Beijing and other regions of the world belonged to the same strain, and we identified the genetic characteristics of G II.12 Norovirus in Beijing.</p>\",\"PeriodicalId\":70973,\"journal\":{\"name\":\"中华实验和临床病毒学杂志\",\"volume\":\"27 3\",\"pages\":\"184-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验和临床病毒学杂志\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验和临床病毒学杂志","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的:了解2008-2009年北京地区成人腹泻患者粪便中分离的GII.12诺如病毒株的遗传特征。方法:采用RT-PCR技术，引物分别扩增RdRp、ORF2、ORF3和ORF1/ORF2重叠区。通过DNAStar、MEGA和SimPlot软件对产物进行纯化、克隆、测序、比对、系统发育和重组分析。结果:经系统发育分析，11株属Gⅱ型。RdRp区域为g, ORF2和ORF3区域为GII.12。SimPlot分析进一步证实11株菌株为重组菌株(Gⅱ)。g [RdRp]/ g II.12[衣壳])。结论:北京地区流行的G II.12诺瓦克病毒与世界其他地区流行的诺瓦克病毒属于同一株，鉴定出北京地区流行的G II.12诺瓦克病毒的遗传特征。
[Genetic characterization of G II.12 norovirus in Beijing from 2008 to 2009].
Objective: To reveal the genetic characteristics of GII.12 Norovirus strains isolating from stool samples of adults with diarrhea in Beijing during 2008-2009.
Methods: RdRp, ORF2, ORF3 and ORF1/ORF2 overlap region were respectively amplified by primers using RT-PCR. The products were purified, cloned, sequenced and then aligned, phylogenetic and recombinant analyzed by softwares of DNAStar, MEGA and SimPlot.
Results: According to the phylogenetic analysis, 11 strains belonged to G II.g in the RdRp region,while GII.12 in the ORF2 and ORF3. SimPlot analysis further confirmed the 11 strains were recombinant strains ( G II.g [RdRp]/G II.12 [capsid]).
Conclusion: G II.12 Norovirus prevailing in Beijing and other regions of the world belonged to the same strain, and we identified the genetic characteristics of G II.12 Norovirus in Beijing.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
