酿酒酵母d -阿拉伯糖脱氢酶Ara1的结构及其与NADPH的复合物:对辅因子辅助底物识别的意义。

IF 0.9 4区 生物学
Xiao-Qian Hu, Peng-Chao Guo, Jin-Di Ma, Wei-Fang Li
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引用次数: 4

摘要

酵母Ara1的主要作用是利用NADPH作为辅助因子,在生理pH水平下催化多种有毒α,β-二羰基化合物的还原,此前被错误地注释为d -阿拉伯糖脱氢酶。在这里,apo和nadph络合形式的Ara1的晶体结构分别以2.10和2.00 Å分辨率呈现。Ara1以同二聚体形式存在,其每个亚基采用(α/β)8桶结构,具有高度保守的辅因子结合袋。结构比较显示,NADPH结合诱导的配合产生了一个完整的活性位点口袋,可以识别底物。此外,结合计算模拟的晶体结构定义了一个开放的底物结合位点,以容纳具有二羰基的各种底物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Structures of Saccharomyces cerevisiae D-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition.

Structures of Saccharomyces cerevisiae D-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition.

Structures of Saccharomyces cerevisiae D-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition.

Structures of Saccharomyces cerevisiae D-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition.

The primary role of yeast Ara1, previously mis-annotated as a D-arabinose dehydrogenase, is to catalyze the reduction of a variety of toxic α,β-dicarbonyl compounds using NADPH as a cofactor at physiological pH levels. Here, crystal structures of Ara1 in apo and NADPH-complexed forms are presented at 2.10 and 2.00 Å resolution, respectively. Ara1 exists as a homodimer, each subunit of which adopts an (α/β)8-barrel structure and has a highly conserved cofactor-binding pocket. Structural comparison revealed that induced fit upon NADPH binding yielded an intact active-site pocket that recognizes the substrate. Moreover, the crystal structures combined with computational simulation defined an open substrate-binding site to accommodate various substrates that possess a dicarbonyl group.

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来源期刊
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期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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