精氨酸加压素通过激活结后V1受体,诱导小鼠远端结肠的收缩作用

Mariangela Mastropaolo, Maria Grazia Zizzo, Michelangelo Auteri, Flavia Mulè, Rosa Serio
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引用次数: 6

摘要

本研究的目的是分析精氨酸抗利尿激素(AVP)是否可能被认为是肠道运动的调节剂。因此,我们在体外评估了外源性AVP对小鼠远端结肠收缩性的影响、激活的受体亚型及其作用机制。对小鼠远端结肠纵肌条和圆形肌条进行等长记录。AVP (0.001 nM - 100 nM)仅对纵肌产生浓度依赖性收缩效应,并被V1受体拮抗剂V-1880拮抗。河豚毒素、阿托品和吲哚美辛对avp的诱导作用无明显影响。在无Ca2 +溶液或硝苯地平的存在下,AVP的收缩反应降低,在无钙培养基中反复添加碳二醇和环吡唑酸后,细胞内钙储存的消耗会消除AVP的收缩反应。磷脂酶C抑制剂U-73122能有效拮抗AVP的作用,而腺苷酸环化酶抑制剂不影响U-73122。催产素在极高浓度时诱导远端结肠纵肌兴奋作用,这种作用可被V-1880拮抗。本研究结果表明,AVP通过激活V1受体,能够独立通过肠神经激活和前列腺素合成,调节小鼠远端结肠纵肌的正收缩活性。收缩反应是通过l型Ca2 +通道的胞外Ca2 +内流和通过磷脂酶C途径从细胞内储存的Ca2 +释放来增加细胞质Ca2 +浓度。未观察到圆形肌的收缩性受到调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Arginine vasopressin, via activation of post-junctional V1 receptors, induces contractile effects in mouse distal colon

The aim of this study was to analyze whether arginine vasopressin (AVP) may be considered a modulator of intestinal motility. In this view, we evaluated, in vitro, the effects induced by exogenous administration of AVP on the contractility of mouse distal colon, the subtype(s) of receptor(s) activated and the action mechanism. Isometric recordings were performed on longitudinal and circular muscle strips of mouse distal colon. AVP (0.001 nM–100 nM) caused concentration-dependent contractile effects only on the longitudinal muscle, antagonized by the V1 receptor antagonist, V-1880. AVP-induced effect was not modified by tetrodotoxin, atropine and indomethacin. Contractile response to AVP was reduced in Ca2 +-free solution or in the presence of nifedipine, and it was abolished by depletion of calcium intracellular stores after repetitive addition of carbachol in calcium-free medium with addition of cyclopiazonic acid. U-73122, an inhibitor of the phospholipase C, effectively antagonized AVP effects, whilst it was not affected by an adenylyl cyclase inhibitor. Oxytocin induced an excitatory effect in the longitudinal muscle of distal colon at very high concentrations, effect antagonized by V-1880. The results of this study shown that AVP, via activation of V1 receptors, is able to modulate positively contractile activity of longitudinal muscle of mouse distal colon, independently by enteric nerve activation and prostaglandin synthesis. Contractile response is achieved by increase in cytoplasmatic Ca2 + concentration via extracellular Ca2 + influx from L-type Ca2 + channels and via Ca2 + release from intracellular stores through phospholipase C pathway. No modulation has been observed on the contractility of the circular muscle.

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来源期刊
Regulatory Peptides
Regulatory Peptides 医学-内分泌学与代谢
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审稿时长
2 months
期刊介绍: Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.
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