GnRH、LH、FSH及其受体在人和大鼠胃肠道中的表达和分布

Elin Sand , Monika Bergvall , Eva Ekblad , Mauro D'Amato , Bodil Ohlsson
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引用次数: 25

摘要

背景:促性腺激素释放激素(GnRH)、促黄体生成素(LH)和促卵泡激素(FSH)调节生殖轴。它们的类似物已被发现影响胃肠道活动和肠神经元存活。本研究旨在探讨GnRH、LH和FSH及其受体在人和大鼠胃肠道中的表达和细胞分布。方法采用生物信息学分析和实时荧光定量PCR技术研究人肠道组织中激素和受体的mRNA表达水平。采用人回肠、结肠和大鼠胃、回肠、结肠全层切片进行免疫细胞化学。抗人神经元蛋白HuC/D抗体(HuC/D)作为一般神经元标志物。评估LH和FSH以及GnRH-、LH-和FSH受体免疫反应(IR)神经元。结果gnrh1 mRNA在小肠和大肠均有表达,而GnRH2主要在小肠中表达。在人回肠和结肠中,大约20%的粘膜下和肌肠神经元显示LH受体免疫反应性。在大鼠中,4%-9%的眼底和回肠肠神经元,13%的粘膜下神经元和21%的结肠肌肠神经元是LH受体- ir。没有检测到LH和FSH的mRNA(人),也没有检测到LH和FSH的完全表达蛋白(人和大鼠),或者GnRH和FSH受体。结论gnrh1和GnRH2 mRNA在人肠道中表达。在人和大鼠的整个胃肠道中都发现了LH受体- ir肠内神经元。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression and distribution of GnRH, LH, and FSH and their receptors in gastrointestinal tract of man and rat

Background

Gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) regulate the reproductive axis. Their analogs have been found to influence gastrointestinal activity and enteric neuronal survival. The aims of the study were to investigate expression and cellular distribution of GnRH, LH, and FSH and their receptors in human and rat gastrointestinal tract.

Methods

Bioinformatic analysis of publicly available microarray gene expression data and Real-Time PCR mRNA quantification were used to study mRNA expression levels of hormones and receptors in human intestinal tissue. Full-thickness sections of human ileum and colon, and rat stomach, ileum, and colon, were used for immunocytochemistry. Antibodies against human neuronal protein HuC/D (HuC/D) were used as general neuronal marker. LH and FSH, and GnRH-, LH-, and FSH receptor immunoreactive (IR) neurons were evaluated.

Results

GnRH1 mRNA was detected in both small and large intestine, whereas GnRH2 was mainly expressed in small intestine. Approximately 20% of both submucous and myenteric neurons displayed LH receptor immunoreactivity in human ileum and colon. In rat, 4%–9% of all enteric neurons in fundus and ileum, and 13% of submucous neurons and 21% of myenteric neurons in colon were LH receptor-IR. Neither mRNA (man) nor the fully expressed proteins (man and rat) of LH and FSH, or GnRH and FSH receptors, could be detected.

Conclusions

GnRH1 and GnRH2 mRNA are expressed in human intestine. LH receptor-IR enteric neurons are found along the entire gastrointestinal tract in both man and rat.

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来源期刊
Regulatory Peptides
Regulatory Peptides 医学-内分泌学与代谢
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审稿时长
2 months
期刊介绍: Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.
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