AGGAG重复中Trans中R-Loop的形成。

IF 1.3 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Nucleic Acids Pub Date : 2013-01-01 Epub Date: 2013-08-26 DOI:10.1155/2013/629218

Kazuya Toriumi, Takuma Tsukahara, Ryo Hanai

{"title":"AGGAG重复中Trans中R-Loop的形成。","authors":"Kazuya Toriumi, Takuma Tsukahara, Ryo Hanai","doi":"10.1155/2013/629218","DOIUrl":null,"url":null,"abstract":"Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling. ","PeriodicalId":16575,"journal":{"name":"Journal of Nucleic Acids","volume":"2013 ","pages":"629218"},"PeriodicalIF":1.3000,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/629218","citationCount":"6","resultStr":"{\"title\":\"R-Loop Formation In Trans at an AGGAG Repeat.\",\"authors\":\"Kazuya Toriumi, Takuma Tsukahara, Ryo Hanai\",\"doi\":\"10.1155/2013/629218\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling. \",\"PeriodicalId\":16575,\"journal\":{\"name\":\"Journal of Nucleic Acids\",\"volume\":\"2013 \",\"pages\":\"629218\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2013-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/2013/629218\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nucleic Acids\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2013/629218\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2013/8/26 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nucleic Acids","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2013/629218","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2013/8/26 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 6

摘要

利用T7 RNA聚合酶转录AGGAG重复序列，研究了RNA- dna杂交或r环的形成。当核糖核酸酶T1存在时，cis中r环的形成减少，表明转录物与模板分离并与模板重新结合。当DNA被超卷曲时，发现转录本在反式中与含有AGGAG重复序列的DNA形成r环。化学修饰的结果表明，负超卷曲作用下，双链在AGGAG重复位点打开。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

R-Loop Formation In Trans at an AGGAG Repeat.

查看原文本刊更多论文

R-Loop Formation In Trans at an AGGAG Repeat.

Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Nucleic Acids BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.10

自引率

21.70%

发文量

审稿时长

12 weeks