AGGAG重复中Trans中R-Loop的形成。

IF 1.3 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Nucleic Acids Pub Date : 2013-01-01 Epub Date: 2013-08-26 DOI:10.1155/2013/629218

Kazuya Toriumi, Takuma Tsukahara, Ryo Hanai

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引用次数: 6

摘要

利用T7 RNA聚合酶转录AGGAG重复序列，研究了RNA- dna杂交或r环的形成。当核糖核酸酶T1存在时，cis中r环的形成减少，表明转录物与模板分离并与模板重新结合。当DNA被超卷曲时，发现转录本在反式中与含有AGGAG重复序列的DNA形成r环。化学修饰的结果表明，负超卷曲作用下，双链在AGGAG重复位点打开。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

R-Loop Formation In Trans at an AGGAG Repeat.

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R-Loop Formation In Trans at an AGGAG Repeat.

Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling.

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来源期刊

Journal of Nucleic Acids BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.10

自引率

21.70%

发文量

审稿时长

12 weeks