{"title":"[在古巴设计和实施a型流感病毒(H1N1)的分子方法]。","authors":"Odalys Valdés Ramírez, Alexander Piñón Ramos, Belsy Acosta Herrera, Clara Savón Valdés, Grehete González Muñoz, Amely Arencibia García, Elías Guilarte García, Guelsys González Báez, Suset Oropeza Fernández, Bárbara Hernández Espinosa, Angel Goyenechea Hernández","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>From March through April of 2009, Mexico notified outbreaks of respiratory illness, due to a new influenza virus of swine origin, which spread over rapidly via human-to-human transmission. The molecular methods currently in use were not suitable because the genome composition based on gene segments of swine, avian and human origin was quite different from the influenza A virus (H1N1) circulating at that time.</p><p><strong>Objective: </strong>Based on the published sequences, a set of specific primers for the HA gene was designed to evaluate a new RT-PCR assay.</p><p><strong>Methods: </strong>The RT-PCR assay processed 3 197 clinical samples from suspected cases of pandemic influenza A (H1N1) infection.</p><p><strong>Results: </strong>The novel optimized method obtained a 262 pb segment, without unspecific reactions. The new method proved to be useful in the diagnosis and subtyping of pandemic HINI influenza virus. The amplified product was verified by nucleotide sequencing, thus confirming the virus.</p><p><strong>Conclusions: </strong>The introduction of this new assay for the laboratory surveillance of influenza virus strengthens the diagnostic capacity of the National Reference Laboratory.</p>","PeriodicalId":35915,"journal":{"name":"Revista Cubana de Medicina Tropical","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Design and implementation of a molecular method for influenza A virus (H1N1) in Cuba].\",\"authors\":\"Odalys Valdés Ramírez, Alexander Piñón Ramos, Belsy Acosta Herrera, Clara Savón Valdés, Grehete González Muñoz, Amely Arencibia García, Elías Guilarte García, Guelsys González Báez, Suset Oropeza Fernández, Bárbara Hernández Espinosa, Angel Goyenechea Hernández\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>From March through April of 2009, Mexico notified outbreaks of respiratory illness, due to a new influenza virus of swine origin, which spread over rapidly via human-to-human transmission. The molecular methods currently in use were not suitable because the genome composition based on gene segments of swine, avian and human origin was quite different from the influenza A virus (H1N1) circulating at that time.</p><p><strong>Objective: </strong>Based on the published sequences, a set of specific primers for the HA gene was designed to evaluate a new RT-PCR assay.</p><p><strong>Methods: </strong>The RT-PCR assay processed 3 197 clinical samples from suspected cases of pandemic influenza A (H1N1) infection.</p><p><strong>Results: </strong>The novel optimized method obtained a 262 pb segment, without unspecific reactions. The new method proved to be useful in the diagnosis and subtyping of pandemic HINI influenza virus. The amplified product was verified by nucleotide sequencing, thus confirming the virus.</p><p><strong>Conclusions: </strong>The introduction of this new assay for the laboratory surveillance of influenza virus strengthens the diagnostic capacity of the National Reference Laboratory.</p>\",\"PeriodicalId\":35915,\"journal\":{\"name\":\"Revista Cubana de Medicina Tropical\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Cubana de Medicina Tropical\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Cubana de Medicina Tropical","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
[Design and implementation of a molecular method for influenza A virus (H1N1) in Cuba].
Introduction: From March through April of 2009, Mexico notified outbreaks of respiratory illness, due to a new influenza virus of swine origin, which spread over rapidly via human-to-human transmission. The molecular methods currently in use were not suitable because the genome composition based on gene segments of swine, avian and human origin was quite different from the influenza A virus (H1N1) circulating at that time.
Objective: Based on the published sequences, a set of specific primers for the HA gene was designed to evaluate a new RT-PCR assay.
Methods: The RT-PCR assay processed 3 197 clinical samples from suspected cases of pandemic influenza A (H1N1) infection.
Results: The novel optimized method obtained a 262 pb segment, without unspecific reactions. The new method proved to be useful in the diagnosis and subtyping of pandemic HINI influenza virus. The amplified product was verified by nucleotide sequencing, thus confirming the virus.
Conclusions: The introduction of this new assay for the laboratory surveillance of influenza virus strengthens the diagnostic capacity of the National Reference Laboratory.
期刊介绍：
La Revista Cubana de Medicina Tropical tiene la misión de publicar artículos científicos especializados en medicina tropical, microbiología, parasitología, epidemiología y otras especialidades afines. Se distribuye directamente por el editor a los suscriptores en formato impreso (ISSN 0375-0760). Está dirigida a profesionales y técnicos en el campo de la medicina tropical, microbiología, parasitología y epidemiología. Recibe contribuciones en idioma español, inglés y portugués sin distinción en el país de procedencia.
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