Liuber Y Machado Zaldivar, Madeline Blanco de Armas, Ana Luisa Lubián Caballero, Héctor M Díaz Torres
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[Use of Amplicor HIV-1 DNA test v.1.5 in diagnosis of perinatal infection from HIV-1 in Cuba].
Introduction: HIV-1 proviral DNA amplification is the preferential method for HIV diagnosis infection in infants and it has been used in Cuba since 1992. AMPLICOR HIV-1 DNA kit is an in vitro qualitative assay for the detection of HIV-1 proviral DNA in the whole blood.
Objective: this paper showed the results of the use of this kit for the first time in Cuba for the perinatal diagnosis of HIV-1 infection.
Methods: three hundred forty six whole blood samples from children of HIV seropositive women were analyzed by the AMPLICOR HIV-1 DNA kit in the period 2005-2007.
Results: among the tested samples, six were positive, and 340 negative.
Conclusions: the assay was reproducible under the Cuban conditions and the achieved results made the diagnosis and follow up of children of HIV-1 seropositive mothers possible.
期刊介绍：
La Revista Cubana de Medicina Tropical tiene la misión de publicar artículos científicos especializados en medicina tropical, microbiología, parasitología, epidemiología y otras especialidades afines. Se distribuye directamente por el editor a los suscriptores en formato impreso (ISSN 0375-0760). Está dirigida a profesionales y técnicos en el campo de la medicina tropical, microbiología, parasitología y epidemiología. Recibe contribuciones en idioma español, inglés y portugués sin distinción en el país de procedencia.
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