[接种抗结核分枝杆菌抗原的“哈瓦那分枝杆菌”小鼠体外干扰素γ的产生]。

Q4 Medicine

Revista Cubana de Medicina Tropical Pub Date : 2012-07-01

Iliana Valdés Hernández, C Ernesto Montoro Cardoso, Rogelio Hernández-Pando

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引用次数: 0

摘要

前言:开发新的抗结核疫苗需要对分枝杆菌抗原诱导的细胞介导的免疫反应进行表征。目的:测定“哈瓦那分枝杆菌”TMC-5135在Balb/c小鼠皮下接种疫苗时的免疫原性。方法:用活的“哈瓦那分枝杆菌”TMC-5135皮下接种Balb/c小鼠。记录用分枝杆菌抗原Ag85b或培养滤液抗原(CFA)刺激后肺、脾和淋巴结的细胞悬液中IFN γ的产生。结果:经CFA和Ag85b刺激后，接种“M. habana”的小鼠IFN γ的产生高于接种卡介苗的小鼠。结论:这些结果鼓励对“M. habana”作为结核病候选疫苗进行新的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

本刊更多论文

[In vitro interferon gamma production in mice vaccinated with 'Mycobacterium habana' against Mycobacterium tuberculosis antigens].

Introduction: development of new antituberculosis vaccines requires the characterization of the cell-mediated immune responses induced by mycobacterial antigens.

Objective: to determine the immunogenic potential of 'Mycobacterium habana' TMC-5135 when using subcutaneous vaccine in Balb/c mice.

Methods: in this study, Balb/c mice were inoculated subcutaneously with live 'Mycobacterium habana' TMC-5135. The production of IFN gamma in cell suspensions obtained from the lungs, the spleen and the lymph nodes after stimulation with mycobacterial antigens Ag85b or culture filtrate antigens (CFA) was recorded.

Results: the production of IFN gamma after stimulation with CFA and Ag85b was higher in mice vaccinated with 'M. habana' than in animals immunized with BCG.

Conclusions: these results encourage new research on 'M. habana' as vaccinal candidate against tuberculosis.

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来源期刊

Revista Cubana de Medicina Tropical Medicine-Infectious Diseases

CiteScore

0.30

自引率

0.00%

发文量

期刊介绍： La Revista Cubana de Medicina Tropical tiene la misión de publicar artículos científicos especializados en medicina tropical, microbiología, parasitología, epidemiología y otras especialidades afines. Se distribuye directamente por el editor a los suscriptores en formato impreso (ISSN 0375-0760). Está dirigida a profesionales y técnicos en el campo de la medicina tropical, microbiología, parasitología y epidemiología. Recibe contribuciones en idioma español, inglés y portugués sin distinción en el país de procedencia.