Aline C Caetano, Lucimara F da Veiga, Flávia R Capaldi, Severino M de Alencar, Ricardo A Azevedo, Rosangela M N Bezerra
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The aim of this study was to evaluate the antioxidant responses of mice to two diets control, commercial and the purified AIN 93 diet, commonly used in experiments with rodents.</p><p><strong>Results: </strong>Malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentrations and superoxide dismutase (SOD) and glutathione reductase (GR) activities determined in the liver were lower in the group of mice fed with the AIN 93 diet, while catalase (CAT) activity was higher in the same group, when compared to the group fed on the commercial diet. Liver glutathione peroxidase (GSH-Px) activity was similar in the groups fed on either AIN 93 or the commercial diets. Two SOD isoforms, Mn-SODII and a Cu/Zn-SODV, were specifically reduced in the liver of the AIN 93 diet fed animals.</p><p><strong>Conclusions: </strong>The clear differences in antioxidant responses observed in the livers of mice fed on the two diets suggest that the macro- and micro-nutrient components with antioxidant properties, including vitamin E, can promote changes in the activity of enzymes involved in the removal of the ROS generated by cell metabolism.</p>","PeriodicalId":35905,"journal":{"name":"BMC Physiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564843/pdf/","citationCount":"0","resultStr":"{\"title\":\"The antioxidant response of the liver of male Swiss mice raised on a AIN 93 or commercial diet.\",\"authors\":\"Aline C Caetano, Lucimara F da Veiga, Flávia R Capaldi, Severino M de Alencar, Ricardo A Azevedo, Rosangela M N Bezerra\",\"doi\":\"10.1186/1472-6793-13-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Reactive oxygen species (ROS) are formed under natural physiological conditions and are thought to play an important role in many human diseases. 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引用次数: 0
摘要
背景:活性氧(ROS)是在自然生理条件下形成的,被认为在许多人类疾病中起着重要作用。多种抗氧化剂参与了细胞抵御 ROS 的机制,这些抗氧化剂包括酶和非酶抗氧化剂。本研究的目的是评估小鼠对两种饮食的抗氧化反应,即啮齿动物实验中常用的对照饮食、商业饮食和纯化 AIN 93 饮食:结果:与使用商品饲料喂养的小鼠相比,使用 AIN 93 饲料喂养的小鼠肝脏中丙二醛(MDA)和过氧化氢(H2O2)浓度以及超氧化物歧化酶(SOD)和谷胱甘肽还原酶(GR)活性较低,而过氧化氢酶(CAT)活性较高。肝脏谷胱甘肽过氧化物酶(GSH-Px)活性在以 AIN 93 或商品饲料喂养的组别中相似。饲喂 AIN 93 的动物肝脏中的两种 SOD 同工酶(Mn-SODII 和 Cu/Zn-SODV )明显减少:结论:在两种饲料喂养的小鼠肝脏中观察到的抗氧化反应的明显差异表明,包括维生素 E 在内的具有抗氧化特性的宏观和微观营养成分可促进参与清除细胞代谢产生的 ROS 的酶的活性发生变化。
The antioxidant response of the liver of male Swiss mice raised on a AIN 93 or commercial diet.
Background: Reactive oxygen species (ROS) are formed under natural physiological conditions and are thought to play an important role in many human diseases. A wide range of antioxidants are involved in cellular defense mechanisms against ROS, which can be generated in excess during stressful conditions, these include enzymes and non-enzymatic antioxidants. The aim of this study was to evaluate the antioxidant responses of mice to two diets control, commercial and the purified AIN 93 diet, commonly used in experiments with rodents.
Results: Malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentrations and superoxide dismutase (SOD) and glutathione reductase (GR) activities determined in the liver were lower in the group of mice fed with the AIN 93 diet, while catalase (CAT) activity was higher in the same group, when compared to the group fed on the commercial diet. Liver glutathione peroxidase (GSH-Px) activity was similar in the groups fed on either AIN 93 or the commercial diets. Two SOD isoforms, Mn-SODII and a Cu/Zn-SODV, were specifically reduced in the liver of the AIN 93 diet fed animals.
Conclusions: The clear differences in antioxidant responses observed in the livers of mice fed on the two diets suggest that the macro- and micro-nutrient components with antioxidant properties, including vitamin E, can promote changes in the activity of enzymes involved in the removal of the ROS generated by cell metabolism.
BMC PhysiologyBiochemistry, Genetics and Molecular Biology-Physiology
CiteScore
9.60
自引率
0.00%
发文量
0
期刊介绍:
BMC Physiology is an open access journal publishing original peer-reviewed research articles in cellular, tissue-level, organismal, functional, and developmental aspects of physiological processes. BMC Physiology (ISSN 1472-6793) is indexed/tracked/covered by PubMed, MEDLINE, BIOSIS, CAS, EMBASE, Scopus, Zoological Record and Google Scholar.