人类子宫内膜干细胞作为神经细胞编程的新来源

Zahra Taherian Mobarakeh, Jafar Ai, Farzad Yazdani, Seyed Mahdi Rezayat Sorkhabadi, Zinat Ghanbari, Abbas Noroozi Javidan, Seyed Abdol Reza Mortazavi-Tabatabaei, Mohammad Massumi, Somayeh Ebrahimi Barough
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引用次数: 60

摘要

人子宫内膜干细胞(EnSC)是一种丰富且容易获得的细胞替代治疗来源。许多研究表明,这些细胞能够分化为几种中胚层来源的细胞系,包括骨细胞和脂肪细胞。本文研究了EnSC在神经分化中的作用。流式细胞术分析显示,CD90、CD105、OCT4、CD44表达阳性,CD31、CD34、CD133表达阴性。分别用碱性成纤维细胞生长因子(bFGF)、血小板衍生生长因子(PDGF)和表皮生长因子(EGF)信号分子诱导细胞向神经分化,并通过逆转录pcr和免疫细胞化学分析分化细胞中神经元标志物的表达,包括Nestin、GABA (γ-氨基丁酸)、MAP2(微管相关蛋白2)、β3-tub (III类β-微管蛋白)和NF-L(神经丝光)的mrna水平。免疫细胞化学方法证实MAP2、β3-tub和NF-L蛋白在enc中的表达。总之,EnSC可以响应通常用作神经分化标准的信号分子,并且可以编程神经细胞,使这些细胞值得考虑作为神经退行性疾病细胞治疗的独特来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Human endometrial stem cells as a new source for programming to neural cells

Human endometrial stem cells as a new source for programming to neural cells

Human EnSC (endometrial-derived stem cell) is an abundant and easily available source for cell replacement therapy. Many investigations have shown the potency of the cells to differentiate into several mesoderm-derived cell lineages, including osteocytes and adipocytes. Here, the potency of EnSC in neural differentiation has been investigated. Flow cytometric analysis showed that they were positive for CD90, CD105, OCT4, CD44 and negative for CD31, CD34, CD133. The characterized cells were induced into neural differentiation by bFGF (basic fibroblast growth factor), PDGF (platelet-derived growth factor) and EGF (epidermal growth factor) signalling molecules, respectively in a sequential protocol, and differentiated cells were analysed for expression of neuronal markers by RT—PCR (reverse transcription—PCR) and immunocytochemistry, including Nestin, GABA (γ-aminobutyric acid), MAP2 (microtubule-associated protein 2), β3-tub (class III β-tubulin) and NF-L (neurofilament-light) at the level of their mRNAs. The expression of MAP2, β3-tub and NF-L proteins in EnSC was confirmed 28 days PT (post-treatment) by immunocytochemistry. In conclusion, EnSC can respond to signalling molecules that are usually used as standards in neural differentiation and can programme neuronal cells, making these cells worth considering as a unique source for cell therapy in neurodegenerative disease.

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