用于瘫痪面部再生的人造肌肉:沙鼠模型的耐久性和生物相容性。

Levi G Ledgerwood, Steven Tinling, Craig Senders, Annjoe Wong-Foy, Harsha Prahlad, Travis T Tollefson
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引用次数: 18

摘要

背景:目前永久性面瘫的治疗主要集中在神经移植和肌肉转移;但是,这些程序的局限性要求有其他选择。目的:测定沙鼠植入人工肌肉的耐久性和生物相容性,以及宿主组织-人工肌肉界面的炎症和纤维化程度。方法:将医用级硅胶材料制成的电活性聚合物人工肌肉(EPAM)装置植入13只沙鼠皮下。通过功能发生器以1 kV、1 Hz、24 h/d的电流刺激植入细胞。记录植入后40天的电信号输入/输出。在植入后23 ~ 65天对动物实施安乐死,对宿主组织-植入物界面进行组织学评价。结果:EPAM植入动物耐受良好,无围手术期死亡。肌肉装置产生运动的平均时间为30.3天(范围19-40天),平均为2.6 × 106个周期(范围1.6 × 106至3.5 × 106个周期)。外植体的组织学检查显示植入物周围有微小的纤维囊,没有细菌感染或炎症浸润的证据。没有证据表明设备损坏,腐蚀或硅胶击穿。结论:该短期动物模型植入人工肌肉是安全的,具有一定的功能性。我们相信,对于不可逆面瘫患者,EPAM装置将是恢复面部运动的一种安全可行的选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Artificial muscle for reanimation of the paralyzed face: durability and biocompatibility in a gerbil model.

Background: Current management of permanent facial paralysis centers on nerve grafting and muscle transfer; however, limitations of those procedures call for other options.

Objectives: To determine the durability and biocompatibility of implanted artificial muscle in a gerbil model and the degree of inflammation and fibrosis at the host tissue-artificial muscle interface.

Methods: Electroactive polymer artificial muscle (EPAM) devices engineered in medical-grade silicone were implanted subcutaneously in 13 gerbils. The implanted units were stimulated with 1 kV at 1 Hz, 24 h/d via a function generator. Electrical signal input/output was recorded up to 40 days after implantation. The animals were euthanized between 23 and 65 days after implantation, and the host tissue-implant interface was evaluated histologically.

Results: The animals tolerated implantation of the EPAM devices well, with no perioperative deaths. The muscle devices created motion for a mean of 30.3 days (range, 19-40 days), with a mean of 2.6 × 106 cycles (range, 1.6 × 106 to 3.5 × 106 cycles). Histologic examination of the explanted devices revealed the development of a minimal fibrous capsule surrounding the implants, with no evidence of bacterial infection or inflammatory infiltrate. No evidence of device compromise, corrosion, or silicone breakdown was noted.

Conclusions: Artificial muscle implanted in this short-term animal model was safe and functional in this preliminary study. We believe that EPAM devices will be a safe and viable option for restoration of facial motions in patients with irreversible facial paralysis.

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