基质金属蛋白酶抑制剂在进展性肾系血管增殖性肾炎中的肾保护作用。

Nephron Extra Pub Date : 2012-01-01 Epub Date: 2012-05-26 DOI:10.1159/000338801
Takayuki Kuroda, Masao Masui, Mitsuru Notoya, Masashi Ito, Yoshinori Tamura, Hiroyuki Okamoto, Eri Kanaoka, Toshihiro Shinosaki
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引用次数: 2

摘要

背景/目的:基质金属蛋白酶(MMPs)在细胞外基质转化中起关键作用,并参与慢性肾脏疾病。一种合成的MMP抑制剂化合物a对慢性肾炎的肾保护作用进行了研究。方法:单侧肾切除大鼠单次注射抗thy1.1抗体诱导肾炎。评价化合物A对蛋白尿、血尿素氮及基质相关基因表达的影响。通过周期性酸希夫染色和羟脯氨酸含量来评估胶原积累。分别用免疫组化蛋白和电镜检测阴离子电荷位点评价肾小球上皮细胞和肾小球基底膜的完整性。结果:复方A治疗可明显减轻蛋白尿,改善血尿素氮,预防肾小球硬化。在治疗的动物中,皮层中胶原蛋白和转化生长因子β1的基因上调被阻止。肾小球上皮细胞损伤较轻,肾小球基底膜阴离子部位受到保护。结论:新型MMP抑制剂化合物A对进行性肾小球肾炎具有保护作用。化合物A改善肾脏损伤的各个方面,可能对肾脏疾病具有治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Renoprotective action of a matrix metalloproteinase inhibitor in progressive mesangioproliferative nephritis.

Renoprotective action of a matrix metalloproteinase inhibitor in progressive mesangioproliferative nephritis.

Renoprotective action of a matrix metalloproteinase inhibitor in progressive mesangioproliferative nephritis.

Renoprotective action of a matrix metalloproteinase inhibitor in progressive mesangioproliferative nephritis.

Background/aim: Matrix metalloproteinases (MMPs) play pivotal roles in extracellular matrix turnover and are involved in chronic kidney disease. The renoprotective action of a synthetic MMP inhibitor, compound A, was investigated in chronic nephritis.

Methods: Nephritis was induced by a single injection of anti-Thy1.1 antibody to unilaterally nephrectomized rats. The effects of compound A on proteinuria, blood urea nitrogen, and matrix-related gene expressions were evaluated. Collagen accumulation, as assessed by periodic acid-Schiff staining and hydroxyproline content, was determined. The integrity of glomerular epithelial cells and glomerular basement membrane was evaluated with desmin immunohistochemistry and electron microscopic detection of anionic charge sites, respectively.

Results: Treatment with compound A notably attenuated proteinuria, ameliorated blood urea nitrogen, and prevented glomerulosclerosis. Gene upregulation of collagen and transforming growth factor β1 in the cortex was prevented in the treated animals. Glomerular epithelial cell injury was milder, and glomerular basement membrane anionic sites were protected with the treatment.

Conclusion: A novel MMP inhibitor, compound A, exerts protective effects in progressive glomerulonephritis. Compound A ameliorates various aspects of renal injuries and may have therapeutic potential toward kidney diseases.

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来源期刊
自引率
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审稿时长
12 weeks
期刊介绍: An open-access subjournal to Nephron. ''Nephron EXTRA'' publishes additional high-quality articles that cannot be published in the main journal ''Nephron'' due to space limitations.
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