将人骨髓和单个核细胞提取物注射到梗死小鼠心脏中,可改善其功能。

Franca S Angeli, Yan Zhang, Richard Sievers, Kristine Jun, Sarah Yim, Andrew Boyle, Yerem Yeghiazarians
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引用次数: 12

摘要

背景:我们之前已经证明,小鼠全骨髓细胞(BMC)提取物可以改善小鼠心肌梗死(MI)模型中缺乏完整细胞的心功能并减少疤痕大小。目前尚不清楚这些结果是否可用于人类BMC (hBMC)或单个核细胞(hMNC)的提取物,这将具有重要的临床意义。方法:以10 ~ 12周龄雄性C57BL/6J小鼠为研究对象。心肌梗死是通过永久性结扎左前降支形成的。动物随机分为三组,分别用hBMCs提取物(n=6)、hMNCs提取物(n=8)和0.5%牛血清白蛋白(n=7)进行超声引导心肌注射。心功能在心肌梗死后的基线、2天和28天通过超声心动图进行评估。心肌梗死后28天评估梗死面积和血管。结果:与对照组相比,hBMC和hMNC提取物能保持心肌功能,心肌梗死后瘢痕大小较小。结论:本研究首次报道了hBMC和hMNC提取物可改善心肌梗死后小鼠心功能。需要进一步的研究来充分说明这些疗法的潜在临床益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Injection of human bone marrow and mononuclear cell extract into infarcted mouse hearts results in functional improvement.

Injection of human bone marrow and mononuclear cell extract into infarcted mouse hearts results in functional improvement.

Injection of human bone marrow and mononuclear cell extract into infarcted mouse hearts results in functional improvement.

Injection of human bone marrow and mononuclear cell extract into infarcted mouse hearts results in functional improvement.

Background: We have previously shown that mouse whole bone marrow cell (BMC) extract results in improvement of cardiac function and decreases scar size in a mouse model of myocardial infarction (MI), in the absence of intact cells. It is not clear if these results are translatable to extracts from human BMC (hBMC) or mononuclear cells (hMNC), which would have significant clinical implications.

Methods: Male C57BL/6J (10-12 weeks old) mice were included in this study. MI was created by permanent ligation of the left anterior descending artery. Animals were randomized into three groups to receive ultrasound-guided myocardial injections with either hBMCs extract (n=6), hMNCs extract (n=8) or control with 0.5% bovine serum albumin (BSA) (n=7). Cardiac function was assessed by echocardiography at baseline, 2 and 28 days post-MI. Infarct size and vascularity was assessed at 28 days post-MI.

Results: hBMC and hMNC extract preserve cardiac function and result in smaller scar size post-MI when compared with the control group.

Conclusions: The current study for the first time reports that hBMC and hMNC extracts improve cardiac function post-MI in a mouse MI model. Further studies are necessary to fully address the potential clinical benefits of these therapies.

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