黄芪多糖可恢复斑马鱼化学诱导的血管损伤。

Q4 Neuroscience
Guang Hu, Gail B Mahady, Shang Li, Maggie Pui Man Hoi, You-Hua Wang, Simon Ming Yuen Lee
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引用次数: 19

摘要

背景:黄芪被广泛用于治疗心脑血管疾病和增强耐力和耐力,在中医中已有2000多年的历史。黄芪多糖(Astragali Radix, ARP)被认为是该药用植物具有多种药理作用的主要成分之一。本研究的目的是在化学诱导的斑马鱼血管丢失模型中评估ARPs的血管再生活性。方法:用300 nM VEGFR酪氨酸激酶抑制剂II (VRI)在24 hpf(受精后小时)下处理3 h,诱导Tg(fli-1a:EGFP)y1和Tg(fli-1a:nEGFP)y7胚胎血管丢失。然后在VRI停药后分别用ARPs处理血管受损斑马鱼21 h和45 h。在荧光显微镜下观察斑马鱼幼体节间血管(ISVs)的形态变化,并进行定量测定。通过实时荧光定量PCR检测Kdrl、Kdr和Flt-1 mRNA的相对表达量,验证ARPs对斑马鱼模型的拯救作用。结果:超滤分离得到黄芪多糖P4 (50000 D <分子量和直径< 0.1 μm)和P5(分子直径> 0.1 μm)对vri诱导的斑马鱼血管损伤有明显的剂量依赖性恢复作用。此外,P4可逆转VRI诱导的Flk-1和Flt-1 mRNA表达下调。结论:从黄芪中分离得到的P4可降低vri诱导的斑马鱼血管损失。这些发现支持了黄芪多糖是黄芪活性成分之一的假设,有助于其对治疗与血管生成不足相关的疾病的有益作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Polysaccharides from astragali radix restore chemical-induced blood vessel loss in zebrafish.

Polysaccharides from astragali radix restore chemical-induced blood vessel loss in zebrafish.

Polysaccharides from astragali radix restore chemical-induced blood vessel loss in zebrafish.

Polysaccharides from astragali radix restore chemical-induced blood vessel loss in zebrafish.

Background: Astragali Radix has been used widely for the treatment of cardiovascular and cerebrovascular diseases, and to enhance endurance and stamina in traditional Chinese medicine (TCM) for over 2000 years. The polysaccharide constituents of Astragali Radix (ARP) are considered as one of the major constituents contributing to the multiple pharmacological effects of this medicinal plant. The purpose of the study is to evaluate the vascular regenerative activities of ARPs in a chemically-induced blood vessel loss model in zebrafish.

Methods: Blood vessel loss was induced in both Tg(fli-1a:EGFP)y1 and Tg(fli-1a:nEGFP)y7 embryos by administration of 300 nM VEGFR tyrosine kinase inhibitor II (VRI) for 3 h at 24 hpf (hour post-fertilization). Then, the blood vessel damaged zebrafish were treated with ARPs for 21 h and 45 h after VRI withdrawal. Morphological changes in intersegmental vessels (ISVs) of zebrafish larvae were observed under the fluorescence microscope and measured quantitatively. The rescue effect of ARPs in the zebrafish models was validated by measuring the relative mRNA expressions of Kdrl, Kdr and Flt-1 using real-time PCR.

Results: Two polysaccharide fractions, P4 (50000 D < molecular weight & diameter < 0.1 μm) and P5 (molecular diameter > 0.1 μm), isolated from Astragali Radix by ultrafiltration, produced a significant and dose-dependent recovery in VRI-induced blood vessel loss in zebrafish. Furthermore, the down-regulation of Flk-1 and Flt-1 mRNA expression induced by VRI was reversed by treatment with P4.

Conclusion: The present study demonstrates that P4 isolated from Astragali Radix reduces VRI-induced blood vessel loss in zebrafish. These findings support the hypothesis that polysaccharides are one of the active constituents in Astragali Radix, contributing to its beneficial effect on treatment of diseases associated with a deficiency in angiogenesis.

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来源期刊
Vascular Cell
Vascular Cell Neuroscience-Neurology
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