Heungbok Kim, Cecelia Webster, Justin K M Roberts, Juthamas Kositsawat, Li-Wei Hung, Thomas C Terwilliger, Chang-Yub Kim
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引用次数: 0
摘要
通过利用 Cibacron Blue F3GA 染料与核苷酸/核苷酸结合蛋白相互作用的能力，确定了与结核分枝杆菌重组蛋白相互作用的配体，并研究了这些配体对结晶的影响。与配体共结晶增强了结晶效果，并使所测试的 10 个蛋白质中的 5 个蛋白质的 X 射线衍射数据的分辨率至少达到 2.7 Å。此外，还从蛋白质与配体的相互作用中获得了有关单个蛋白质功能的线索。
Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.
Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands.
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