造血细胞向内皮细胞分化的体外研究。

Bone Marrow Research Pub Date : 2011-01-01 Epub Date: 2011-12-29 DOI:10.1155/2011/846096
Qi Ru Wang, Bao He Wang, Wen Biao Zhu, Yan Hong Huang, Yi Li, Qi Yan
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引用次数: 13

摘要

骨髓来源的内皮祖细胞(BM-EPCs)有助于出生后新生血管的形成,因此对缺血性疾病的细胞治疗有很大的兴趣。然而,它们的起源和特征仍然存在争议。在本文中,我们鉴定了从骨髓单个核细胞中分离并在骨髓内皮细胞条件培养基(ECCM)诱导下分化的BM-EPCs的来源/谱系。BM-EPCs在表型、谱系潜力和功能特性方面进行了表征。用ECCM培养BM-EPC衍生的内皮细胞集落3个月。体外培养的EPC集落细胞表达内皮细胞标志物,并形成毛细血管样网络。EPC集落细胞表达差异增殖能力;一些菌落表现出高增殖潜力(HPP)能力,可达20倍的种群。更重要的是,这些HPP-EPCs表达造血标志物CD45,表现出内吞活性,并保留了一些髓细胞活性。此外,HPP-EPCs在培养基中分泌多种生长因子,包括VEGF和GM-CSF。结果表明,这些EPCs主要来源于早期前体细胞的造血来源,并保持了较高的增殖潜能,这一特征在缺血性疾病的细胞治疗中具有重要的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An in vitro study of differentiation of hematopoietic cells to endothelial cells.

An in vitro study of differentiation of hematopoietic cells to endothelial cells.

An in vitro study of differentiation of hematopoietic cells to endothelial cells.

An in vitro study of differentiation of hematopoietic cells to endothelial cells.

Bone-marrow-derived endothelial progenitor cells (BM-EPCs) contribute to postnatal neovascularization and therefore are of great interest for cell therapies to treat ischemic diseases. However, their origin and characteristics are still in controversy. In this paper, we identified the origin/lineage of the BM-EPCs that were isolated from bone marrow mononuclear cells and differentiated with the induction of bone-marrow endothelial-cellconditioned medium (ECCM). BM-EPCs were characterized in terms of phenotype, lineage potential, and their functional properties. Endothelial cell colonies derived from BM-EPC were cultured with ECCM for 3 months. Cultured EPC colony cells expressed endothelial cell markers and formed the capillary-like network in vitro. EPC colony cells expressed differential proliferative capacity; some of the colonies exhibited a high proliferative potential (HPP) capacity up to 20 population doublings. More importantly, these HPP-EPCs expressed hematopoietic marker CD45, exhibited endocytic activities, and preserved some of the myeloid cell activity. In addition, the HPP-EPCs secrete various growth factors including VEGF and GM-CSF into the culture medium. The results demonstrate that these EPCs were primarily derived from hematopoietic origin of early precursor cells and maintained high proliferative potential capacity, a feature with a significant potential in the application of cell therapy in ischemic diseases.

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