Effect of insulin plant leaves on dexamethasone-induced hyperglycemia.

The material and methods section has following fl aws: • Authentication of plant leaves: As per current policy, it should be carried out from an authentic Institution and not from an Ayurvedic practitioner. • Glibenclamide: Ideally pure compound should have been used than a commercial preparation. • Dose and duration of dexamethasone: Dexamethasone in the dose of 10 mg/kg/day was used for studying lipid content in rats. Dexamethasone has been used as 1 mg/ kg for 22 days to induce diabetes mellitus in rats.[2] Dexamethasone 15 mg/kg/day for 24 days causes insulin resistance in 100% of rats, but induces diabetes mellitus only in 16% of rats.[3] There is no reference for use of dexamethasone for 20 days at dose of 10 mg/kg/day. • Glucose load: As per standard books, glucose load is 1 gm/kg PO; whereas the dose used in study is 2.5 gm/kg PO. The reference for this dose of glucose is not given.[4] • Timing of glucose estimation: Glucose from retro-orbital blood was measured only once at 1 hour. Ideally, it has to be at 1, 2, 4, 8 and 24 hours.[4] • Selection of model: Glucocorticoids induce insulin resistance and glibenclamide is used as positive control. Metformin/ Pioglitazone would have been an ideal comparator or streptozotocin-induced hypoglycemia would be an ideal model for using glibenclamide as positive control.[5] • Mortality of rats: Exact number of rats along with cause of death should be mentioned. Whether the insulin plant leaves caused death due to toxicity or hypoglycemia or whether steroids lead to immunosuppression and infection and caused mortality cannot be deciphered.