大规模克隆和合成基因组的出现。

Lise Goltermann, Thomas Bentin
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引用次数: 2

摘要

分子生物学在生物科学中的突出作用要归功于重组DNA的工具。虽然重组DNA的基础是在20世纪70年代随着II型限制性内切酶的发现,强大的测序技术的发展和基因合成的开创性工作奠定的,但直到新千年之交,第一个完整的合成病毒基因组才出现,包括丙型肝炎,脊髓灰质炎病毒,噬菌体PhiX174.8。随着整个细胞基因组的测序和数字化存储,重组DNA已经成熟信息。那么下一步是什么呢?重组DNA的一个新分支,被称为合成基因组学,9是利用虚拟序列信息和化学成分(重新)构建整个细胞基因组。在这里,我们来看看这种从头开始的建筑的最新发展。要对基因组工程进行更广泛和更广泛的回顾,请参阅卡尔和丘奇的优秀论文。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mega-cloning and the advent of synthetic genomes.

Mega-cloning and the advent of synthetic genomes.

Molecular biology owes its prominent role in the biological sciences to the tools of recombinant DNA. While the foundations of recombinant DNA were laid in the 1970s with the discovery of type II restriction endonucleases,1,2 development of robust sequencing technology3 and pioneering work on gene synthesis,4,5 it was not until the turn of the new millennium before the first complete synthetic viral genomes saw the light of day including that of hepatitis C,6 poliovirus,7 and bacteriophage PhiX174.8 Recombinant DNA has come of age as entire cellular genomes are sequenced and stored as digitized information. So what's next? One novel branch of recombinant DNA, referred to as synthetic genomics,9 is occupied with (re)construction of entire cellular genomes from virtual sequence information and using chemical components. Here we look at the most recent developments in such de novo construction. For a broader and more extensive review on genome engineering, the reader is referred to the excellent paper by Carr and Church.10.

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