{"title":"细胞内氯离子通道1在内皮细胞生长和迁移中起重要作用。","authors":"Jennifer J Tung, Jan Kitajewski","doi":"10.1186/2040-2384-2-23","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Little is known about the role of CLIC1 in endothelium. These studies investigate CLIC1 as a regulator of angiogenesis by in vitro techniques that mimic individual steps in the angiogenic process.</p><p><strong>Methods: </strong>Using shRNA against clic1, we determined the role of CLIC1 in primary human endothelial cell behavior.</p><p><strong>Results: </strong>Here, we report that reduced CLIC1 expression caused a reduction in endothelial migration, cell growth, branching morphogenesis, capillary-like network formation, and capillary-like sprouting. FACS analysis showed that CLIC1 plays a role in regulating the cell surface expression of various integrins that function in angiogenesis including β1 and α3 subunits, as well as αVβ3 and αVβ5.</p><p><strong>Conclusions: </strong>Together, these results indicate that CLIC1 is required for multiple steps of in vitro angiogenesis and plays a role in regulating integrin cell surface expression.</p>","PeriodicalId":88540,"journal":{"name":"Journal of angiogenesis research","volume":"2 ","pages":"23"},"PeriodicalIF":0.0000,"publicationDate":"2010-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/2040-2384-2-23","citationCount":"62","resultStr":"{\"title\":\"Chloride intracellular channel 1 functions in endothelial cell growth and migration.\",\"authors\":\"Jennifer J Tung, Jan Kitajewski\",\"doi\":\"10.1186/2040-2384-2-23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Little is known about the role of CLIC1 in endothelium. These studies investigate CLIC1 as a regulator of angiogenesis by in vitro techniques that mimic individual steps in the angiogenic process.</p><p><strong>Methods: </strong>Using shRNA against clic1, we determined the role of CLIC1 in primary human endothelial cell behavior.</p><p><strong>Results: </strong>Here, we report that reduced CLIC1 expression caused a reduction in endothelial migration, cell growth, branching morphogenesis, capillary-like network formation, and capillary-like sprouting. FACS analysis showed that CLIC1 plays a role in regulating the cell surface expression of various integrins that function in angiogenesis including β1 and α3 subunits, as well as αVβ3 and αVβ5.</p><p><strong>Conclusions: </strong>Together, these results indicate that CLIC1 is required for multiple steps of in vitro angiogenesis and plays a role in regulating integrin cell surface expression.</p>\",\"PeriodicalId\":88540,\"journal\":{\"name\":\"Journal of angiogenesis research\",\"volume\":\"2 \",\"pages\":\"23\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1186/2040-2384-2-23\",\"citationCount\":\"62\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of angiogenesis research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/2040-2384-2-23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of angiogenesis research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/2040-2384-2-23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Chloride intracellular channel 1 functions in endothelial cell growth and migration.
Background: Little is known about the role of CLIC1 in endothelium. These studies investigate CLIC1 as a regulator of angiogenesis by in vitro techniques that mimic individual steps in the angiogenic process.
Methods: Using shRNA against clic1, we determined the role of CLIC1 in primary human endothelial cell behavior.
Results: Here, we report that reduced CLIC1 expression caused a reduction in endothelial migration, cell growth, branching morphogenesis, capillary-like network formation, and capillary-like sprouting. FACS analysis showed that CLIC1 plays a role in regulating the cell surface expression of various integrins that function in angiogenesis including β1 and α3 subunits, as well as αVβ3 and αVβ5.
Conclusions: Together, these results indicate that CLIC1 is required for multiple steps of in vitro angiogenesis and plays a role in regulating integrin cell surface expression.
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