[南宁市散在急性胃肠炎病例中检出诺如病毒I.8基因型]。

中国疫苗和免疫 Pub Date : 2010-06-01
Li-li Deng, Wei Liu, Dong-mei Tan
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引用次数: 0

摘要

目的:研究南宁市某医院散发性肠胃炎患者诺如病毒I.8基因型的基因特征。方法:收集2007年1月~ 2008年12月南宁市某医院腹泻患者标本,采用实时逆转录聚合酶链反应(real-time RT-PCR)检测诺如病毒基因群核糖核酸(RNA)。所有阳性标本进行测序分析,构建系统发育树。结果:696份粪便标本中3份检出诺如病毒I基因组RNA。其中1株(NN07230)与G I.8参考菌株(WUG I/00/JP)同源性为94.5%;NN07230株与2006-2007年日本、韩国流行株同源性为96.7% ~ 98.5%。它们在系统发育树上形成了一个集群。结论:南宁市散发性胃肠炎中存在I.8基因型感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Norovrius genotype I.8 being detected from sporadic cases of acute gastroenteritis in Nanning municipal].

Objective: To study the genic characteristic of Norovrius genotype I.8 from sporadic gastroenteritis of a hospital in Nanning Municipal.

Methods: Specimens of diarrhea patients from a hospital in Nanning Municipal between January 2007 and December 2008 were collected and detected for Noroviral genogroup Iribonucleic acid (RNA) by real-time reverse transcription-polymerase chain reaction(real-time RT-PCR). All positive specimens were subjected for sequencing analysis and constructed phylogenetic tree.

Results: Norovirus genogroup I RNA were detected in 3 of 696 stool specimens. The homology between one of the four strains (NN07230) and reference strain of G I.8 (WUG I/00/JP) was 94.5%; and the homology between the NN07230 strain and strains circulating during 2006-2007 in Japan and Korea ranged from 96.7% to 98.5%. And they formed one cluster in the phylogenetic tree.

Conclusion: The research showed that genotype I.8 infection exists in sporadic gastroenteritis in Nanning City.

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