{"title":"抗癌有机金属钌化合物[(α -p- cymene)Ru(ATSC)Cl]PF(6)与人血清白蛋白的反应。","authors":"Floyd A Beckford","doi":"10.1155/2010/975756","DOIUrl":null,"url":null,"abstract":"<p><p>The reaction of [(eta(6)-p-cymene)Ru(ATSC)Cl]PF(6) (ATSC = 9-anthraldehyde thiosemicarbazone) with human serum albumin was investigated at different temperatures using fluorescence and infrared spectrophotometry. The binding constant, K, for the reaction was determined using a number of different methods. Using a modified Stern-Volmer equation, K was determined to be 9.09 x 10(4), 12.1 x 10(4), and 13.1 x 10(4) M(-1) at 293 K, 298 K, and 308 K, respectively. A thermodynamic analysis showed that the reaction is spontaneous with DeltaG being negative. The enthalpy of reaction DeltaH = 16.5kJ mol(-1) and the entropy of reaction DeltaS = 152 Jmol(-1)K(-1). The values of DeltaH and DeltaS suggest that hydrophobic forces are dominant in the mode of interaction and that the process is mostly entropy driven.</p>","PeriodicalId":14074,"journal":{"name":"International Journal of Inorganic Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2010/975756","citationCount":"24","resultStr":"{\"title\":\"Reaction of the Anticancer Organometallic Ruthenium Compound, [(eta-p-Cymene)Ru(ATSC)Cl]PF(6) with Human Serum Albumin.\",\"authors\":\"Floyd A Beckford\",\"doi\":\"10.1155/2010/975756\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The reaction of [(eta(6)-p-cymene)Ru(ATSC)Cl]PF(6) (ATSC = 9-anthraldehyde thiosemicarbazone) with human serum albumin was investigated at different temperatures using fluorescence and infrared spectrophotometry. The binding constant, K, for the reaction was determined using a number of different methods. Using a modified Stern-Volmer equation, K was determined to be 9.09 x 10(4), 12.1 x 10(4), and 13.1 x 10(4) M(-1) at 293 K, 298 K, and 308 K, respectively. A thermodynamic analysis showed that the reaction is spontaneous with DeltaG being negative. The enthalpy of reaction DeltaH = 16.5kJ mol(-1) and the entropy of reaction DeltaS = 152 Jmol(-1)K(-1). The values of DeltaH and DeltaS suggest that hydrophobic forces are dominant in the mode of interaction and that the process is mostly entropy driven.</p>\",\"PeriodicalId\":14074,\"journal\":{\"name\":\"International Journal of Inorganic Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/2010/975756\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Inorganic Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2010/975756\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Inorganic Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2010/975756","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 24
摘要
采用荧光和红外分光光度法研究了[(eta(6)-对伞花素)Ru(ATSC)Cl]PF(6) (ATSC = 9-蒽醛硫代氨基脲)与人血清白蛋白在不同温度下的反应。反应的结合常数K是用许多不同的方法测定的。使用改进的Stern-Volmer方程,在293 K, 298 K和308 K下,K分别为9.09 x 10(4), 12.1 x 10(4)和13.1 x 10(4) M(-1)。热力学分析表明,该反应为自发反应,DeltaG为负。反应焓δ δ = 16.5kJ mol(-1),熵δ δ = 152 Jmol(-1)K(-1)。δ δ和δ δ的值表明疏水力在相互作用模式中占主导地位,并且该过程主要是熵驱动的。
Reaction of the Anticancer Organometallic Ruthenium Compound, [(eta-p-Cymene)Ru(ATSC)Cl]PF(6) with Human Serum Albumin.
The reaction of [(eta(6)-p-cymene)Ru(ATSC)Cl]PF(6) (ATSC = 9-anthraldehyde thiosemicarbazone) with human serum albumin was investigated at different temperatures using fluorescence and infrared spectrophotometry. The binding constant, K, for the reaction was determined using a number of different methods. Using a modified Stern-Volmer equation, K was determined to be 9.09 x 10(4), 12.1 x 10(4), and 13.1 x 10(4) M(-1) at 293 K, 298 K, and 308 K, respectively. A thermodynamic analysis showed that the reaction is spontaneous with DeltaG being negative. The enthalpy of reaction DeltaH = 16.5kJ mol(-1) and the entropy of reaction DeltaS = 152 Jmol(-1)K(-1). The values of DeltaH and DeltaS suggest that hydrophobic forces are dominant in the mode of interaction and that the process is mostly entropy driven.