一种以琼脂糖凝胶为基础的细胞系运输方法。

Lingzhi Yang, Chufang Li, Ling Chen, Zhiyuan Li
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引用次数: 15

摘要

冷冻保存细胞储存在干冰或液氮中是在世界各地不同城市的研究实验室之间运输细胞以保持细胞活力的经典方法。另一种方法是将活细胞装在充满细胞培养基的烧瓶中。这两种方法都有局限性,要么需要特殊的运输容器,要么细胞在运输过程中存活的时间短。我们最近开发了一种基于琼脂糖凝胶的方法,用于在室温下直接运输细胞培养板或培养皿中的活贴壁细胞。这种方便的方法简化了活细胞的长距离运输,可以使细胞在几天内保持良好的活力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An agarose-gel based method for transporting cell lines.

An agarose-gel based method for transporting cell lines.

An agarose-gel based method for transporting cell lines.

An agarose-gel based method for transporting cell lines.

Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability. An alternative method is to ship the live cells in flasks filled with cell culture medium. Both methods have limitations of either a requirement on special shipping container or short times for the cells to survive on the shipping process. We have recently developed an agarose gel based method for directly transporting the live adherent cells in cell culture plates or dishes in ambient temperature. This convenient method simplifies the transportation of live cells in long distance that can maintain cells in good viability for several days.

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