鸡对沙门氏菌和弯曲杆菌感染的抗性选择。

P Kaiser, M M J Howell, M Fife, J-R Sadeyen, N Salmon, L Rothwell, J Young, T-Y Poh, M Stevens, J Smith, D Burt, C Swaggerty, M Kogut
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引用次数: 0

摘要

对肠道病原体(如沙门氏菌和弯曲杆菌)感染的耐药性可以在许多层面上,包括非免疫机制和免疫机制。免疫抵抗机制可以是特异性的,在适应性免疫反应水平上,或非特异性的,在先天免疫反应水平上。虽然我们可以从已知的哺乳动物对这些病原体的免疫反应在一定程度上推断鸟类,但鸡不是“长羽毛的老鼠”,但与哺乳动物相比,鸡在免疫反应中具有不同的基因、分子、细胞和器官。因此,鸡对肠道病原体的免疫反应的基础工作仍然需要。我们的研究重点是先天免疫反应,因为来自商业鸟类的嗜中性粒细胞(禽类中性粒细胞等量)和来自鸡自交系的巨噬细胞的反应与各种沙门氏菌血清型和感染模型对沙门氏菌感染的抗性或易感性相关。我们研究两种基本的耐药机制——对沙门氏菌或弯曲杆菌定植的耐药性,以及对系统性沙门氏菌病(或禽伤寒)的耐药性。为了定位与沙门氏菌和弯曲杆菌定植抗性相关的基因,我们使用了来自微阵列研究的表达数量性状位点(eQTLs)、全基因组SNP阵列(WGA)、候选基因方法和基因组拷贝数变化分析的组合。为了对系统性沙门氏菌病产生抗性,我们利用高密度SNP面板,结合对抗性和易感品系进行先进回交,确定了5号染色体上一个新的抗性位点SAL1的位置。利用第6代回交定位群体,我们确定并完善了SAL1位点在第5染色体上的8-00 kb。这个区域跨越14个基因,包括两个非常引人注目的功能性候选基因;cd27结合蛋白(Siva)和rac - α丝氨酸/苏氨酸蛋白激酶同源物AKT1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Towards the selection of chickens resistant to Salmonella and Campylobacter infections.

Resistance to infection with enteric pathogens such as Salmonella and Campylobacter can be at many levels and include both non-immune and immune mechanisms. Immune resistance mechanisms can be specific, at the level of the adaptive immune response, or non-specific, at the level of the innate immune response. Whilst we can extrapolate to some degree in birds from what is known about immune responses to these pathogens in mammals, chickens are not "feathered mice", but have a different repertoire of genes, molecules, cells and organs involved in their immune response compared to mammals. Fundamental work on the chicken's immune response to enteric pathogens is therefore still required. Our studies focus particularly on the innate immune response, as responses of heterophils (the avian neutrophil equivalent) from commercial birds, and macrophages from inbred lines of chickens, correlate with resistance or susceptibility to Salmonella infection with a variety of Salmonella serovars and infection models. We work on two basic resistance mechanisms - resistance to colonization with Salmonella or Campylobacter, and resistance to systemic salmonellosis (or fowl typhoid). To map genes involved in resistance to colonization with Salmonella and Campylobacter, we are using a combination of expression quantitative trait loci (eQTLs) from microarray studies, allied with whole genome SNP arrays (WGA), a candidate gene approach and analysis of copy number variation across the genome. For resistance to systemic salmonellosis, we have refined the location ofa novel resistance locus on Chromosome 5, designated SAL1, using high density SNP panels, combined with advanced back-crossing of resistant and susceptible lines. Using a 6th generation backcross mapping population we have confirmed and refined the SAL1 locus to 8-00 kb of Chromosome 5. This region spans 14 genes, including two very striking functional candidates; CD27-binding protein (Siva) and the RAC-alpha serine/threonine protein kinase homologue, AKT1.

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