组织工程中的细胞引导:SDF-1介导间充质干细胞在三维聚己内酯支架中的定向归巢。

Jan-Thorsten Schantz, Harvey Chim, Matthew Whiteman
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引用次数: 105

摘要

细胞引导是一种新的组织工程概念,旨在实现不需要细胞播种的全体内组织工程。这项技术旨在通过不断向植入支架的不同区域递送细胞因子来创造仿生环境,从而实现细胞的位点特异性归巢。在本研究中,通过免疫组织化学和流式细胞术表征了CXCR4在间充质干细胞(MSCs)上的表达,随后证实了对基质细胞衍生因子1 (SDF-1)的趋化性。在随后的三维体外研究中,MSCs在响应SDF-1的情况下在聚己内酯支架内迁移,从而实现极化组织的形成。一个定制的细胞因子微递送系统包括一个储层外壳系统和微针装置,以确保SDF-1持续递送到支架。在此实验基础上,我们在活体大鼠骨组织工程模型中证明,通过微针装置每隔10天递送一种由血管内皮生长因子、SDF-1和骨形态发生蛋白-6组成的细胞因子组合,可以通过迁移细胞前沿导致组织形成,并证明血管生成和血管形成,而无需在植入前在支架上播种细胞。总之,细胞引导为组织工程提供了细胞方法学的进步,并承诺为组织再生提供一种新颖的、微创的选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cell guidance in tissue engineering: SDF-1 mediates site-directed homing of mesenchymal stem cells within three-dimensional polycaprolactone scaffolds.

Cell guidance is a new tissue engineering concept aimed at total in vivo tissue engineering without the need for cell seeding. This technique aims to create a biomimetic environment through constant delivery of cytokines to different areas of an implanted scaffold, such that site-specific homing of cells can be achieved. In this study, expression of CXCR4 on mesenchymal stem cells (MSCs) was characterized by immunohistochemistry and flow cytometry, subsequent to which chemotaxis toward stromal cell-derived factor 1 (SDF-1) was demonstrated. In a subsequent three-dimensional in vitro study, MSCs were shown to migrate within a polycaprolactone scaffold in response to SDF-1, such that polarized tissue formation could be achieved. A customized cytokine microdelivery system comprising a reservoir housing system and microneedle apparatus was fabricated to ensure constant delivery of SDF-1 to the scaffold. Following on this experiment, we demonstrated in an in vivo rat bone tissue engineering model that a cytokine combination consisting of vascular endothelial growth factor, SDF-1, and bone morphogenetic protein-6 delivered at 10-day intervals through the microneedle apparatus could lead to tissue formation through migrating cell fronts, with evidence of angiogenesis and vascularization without the need for cell seeding on scaffolds prior to implantation. In summary, cell guidance offers an advancement in cellular methodology for tissue engineering, and promises a novel, minimally invasive option for tissue regeneration.

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来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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