丙烯腈基聚合物膜用于人角质形成细胞的选择性培养。

Gregor Boese, Christiane Trimpert, Wolfgang Albrecht, Günter Malsch, Thomas Groth, Andreas Lendlein
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引用次数: 19

摘要

利用人工合成材料制备支持角质形成细胞选择性生长的细胞载体是避免皮肤替代物体外培养过程中成纤维细胞过度生长现象的一种很有前途的方法。因此,我们研究了由聚丙烯腈和丙烯腈与n -乙烯基吡咯烷酮(NVP)共聚物制成的聚合物膜对角质形成细胞选择性生长的支持能力。研究发现,在含血清条件下,nvp含量为30%的共聚物(NVP30)支持人角化细胞细胞系(HaCaT)细胞的生长,抑制成纤维细胞的生长。观察HaCaT细胞在14 d内的增殖情况。如果两种类型的细胞在无血清条件下培养,在这些NVP30聚合物上初始粘附超过6小时,它们的粘附程度相同。两种细胞共培养进行了超过7天的长期实验,结果表明HaCaT细胞具有生长优势,这似乎与污染成纤维细胞的旁分泌活性有关。结果,HaCaT细胞与少量剩余的成纤维细胞形成融合层。新型聚丙烯腈-co(NVP)膜是一种很有前途的表皮移植培养体系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Membranes from acrylonitrile-based polymers for selective cultivation of human keratinocytes.

A cell carrier made from synthetic material supporting selective growth of keratinocytes is a promising approach to avoid the phenomenon of fibroblast overgrowth during in vitro culture of skin substitutes. Therefore, we investigated polymer membranes made of polyacrylonitrile and copolymers of acrylonitrile and N-vinylpyrrolidone (NVP) for their ability to support selectively the growth of keratinocytes. It was found that a copolymer with an NVP-content of 30% (NVP30) supports growth of human keratinocyte cell line (HaCaT) cells and inhibits fibroblast growth under serum-containing conditions. Cell proliferation of HaCaT cells was measured over 14 days. If both cell types were cultured under serum-free conditions for initial adhesion over 6 h on these NVP30 polymers, they adhered to the same extent. Long-term experiments over 7 days were performed as a coculture of both cell types showing that HaCaT cells had a growth advantage that seems to be related to the paracrine activity of contaminating fibroblasts. As a result, confluent layers of HaCaT cells were obtained with small numbers of remaining fibroblasts. The new poly [acrylonitrile-co(NVP) membranes seem to be a promising culture system for the production of epidermal transplants.

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Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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