血管平滑肌细胞的表型可塑性——体外和体内剪应力对血管组织工程的影响。

Florian Opitz, Katja Schenke-Layland, Tina U Cohnert, Ulrich A Stock
{"title":"血管平滑肌细胞的表型可塑性——体外和体内剪应力对血管组织工程的影响。","authors":"Florian Opitz,&nbsp;Katja Schenke-Layland,&nbsp;Tina U Cohnert,&nbsp;Ulrich A Stock","doi":"10.1089/ten.2006.0424","DOIUrl":null,"url":null,"abstract":"<p><p>Vascular smooth muscle cells (vSMCs) can switch between a contractile (differentiated) and a synthetic (dedifferentiated) phenotype. Synthetic, proliferative vSMCs are observed during embryogenesis, wound repair, and tissue engineering. The potential of isolated vSMCs to reverse this phenotypic modulation depends strictly on culture conditions. Previous studies have demonstrated that applied shear stress is an important signal for vSMC phenotype. The objective of this study was to determine whether applied shear stress is capable of triggering re-differentiation of vSMCs in tissue-engineered aortas. vSMCs were isolated from ovine arteries. Cells were cultured statically or exposed to two- (2D) and three-dimensional (3D) shear stress after seeding on a tubular matrix. For 3D in vivo testing, grafts were seeded additionally with endothelial cells and implanted in the descending aorta. Particular attention was paid to the expression pattern of vSMC markers, cell ultra-structure, matrix remodeling activity, and proliferative activity. Cultured vSMCs de-differentiated during static in vitro culture, but 2D and 3D in vitro shear stress promoted re-expression of vSMC markers. During in vivo culture, vSMCs progressed toward a fully differentiated phenotype. Cells were expressing markers of differentiated vSMCs and resembled a morphologically contractile vSMC phenotype. Matrix remodeling activity and proliferative activity decreased. This study demonstrates the phenotypic plasticity of vSMCs and their ability to return to a differentiated phenotype under shear stress conditions. These results are crucial for tissue engineering of blood vessels, because they indicate for the first time the in vitro potential to regain physiological functionality of isolated vSMCs.</p>","PeriodicalId":23102,"journal":{"name":"Tissue engineering","volume":"13 10","pages":"2505-14"},"PeriodicalIF":0.0000,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/ten.2006.0424","citationCount":"42","resultStr":"{\"title\":\"Phenotypical plasticity of vascular smooth muscle cells-effect of in vitro and in vivo shear stress for tissue engineering of blood vessels.\",\"authors\":\"Florian Opitz,&nbsp;Katja Schenke-Layland,&nbsp;Tina U Cohnert,&nbsp;Ulrich A Stock\",\"doi\":\"10.1089/ten.2006.0424\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Vascular smooth muscle cells (vSMCs) can switch between a contractile (differentiated) and a synthetic (dedifferentiated) phenotype. Synthetic, proliferative vSMCs are observed during embryogenesis, wound repair, and tissue engineering. The potential of isolated vSMCs to reverse this phenotypic modulation depends strictly on culture conditions. Previous studies have demonstrated that applied shear stress is an important signal for vSMC phenotype. The objective of this study was to determine whether applied shear stress is capable of triggering re-differentiation of vSMCs in tissue-engineered aortas. vSMCs were isolated from ovine arteries. Cells were cultured statically or exposed to two- (2D) and three-dimensional (3D) shear stress after seeding on a tubular matrix. For 3D in vivo testing, grafts were seeded additionally with endothelial cells and implanted in the descending aorta. Particular attention was paid to the expression pattern of vSMC markers, cell ultra-structure, matrix remodeling activity, and proliferative activity. Cultured vSMCs de-differentiated during static in vitro culture, but 2D and 3D in vitro shear stress promoted re-expression of vSMC markers. During in vivo culture, vSMCs progressed toward a fully differentiated phenotype. Cells were expressing markers of differentiated vSMCs and resembled a morphologically contractile vSMC phenotype. Matrix remodeling activity and proliferative activity decreased. This study demonstrates the phenotypic plasticity of vSMCs and their ability to return to a differentiated phenotype under shear stress conditions. These results are crucial for tissue engineering of blood vessels, because they indicate for the first time the in vitro potential to regain physiological functionality of isolated vSMCs.</p>\",\"PeriodicalId\":23102,\"journal\":{\"name\":\"Tissue engineering\",\"volume\":\"13 10\",\"pages\":\"2505-14\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1089/ten.2006.0424\",\"citationCount\":\"42\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tissue engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/ten.2006.0424\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/ten.2006.0424","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 42

摘要

血管平滑肌细胞(vSMCs)可以在收缩(分化)和合成(去分化)表型之间切换。在胚胎发生、伤口修复和组织工程中,可以观察到人工合成的、增殖的vsmc。分离的vSMCs逆转这种表型调节的潜力严格取决于培养条件。以往的研究表明,施加剪切应力是vSMC表型的重要信号。本研究的目的是确定施加剪切应力是否能够触发组织工程主动脉中vSMCs的再分化。从绵羊动脉中分离vSMCs。细胞在管状基质上播种后,静态培养或暴露于二维和三维剪切应力下。在三维体内实验中,移植物被另外植入内皮细胞并植入降主动脉。特别关注vSMC标志物的表达模式、细胞超微结构、基质重塑活性和增殖活性。体外静态培养时,体外培养的vSMC去分化,但体外2D和3D剪切应力促进了vSMC标志物的重新表达。在体内培养过程中,vSMCs向完全分化表型发展。细胞表达分化的vSMC标记物,形态上类似于收缩的vSMC表型。基质重塑活性和增殖活性降低。本研究证明了vSMCs的表型可塑性及其在剪切应力条件下恢复分化表型的能力。这些结果对于血管的组织工程是至关重要的,因为它们首次表明了分离的vSMCs在体外恢复生理功能的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phenotypical plasticity of vascular smooth muscle cells-effect of in vitro and in vivo shear stress for tissue engineering of blood vessels.

Vascular smooth muscle cells (vSMCs) can switch between a contractile (differentiated) and a synthetic (dedifferentiated) phenotype. Synthetic, proliferative vSMCs are observed during embryogenesis, wound repair, and tissue engineering. The potential of isolated vSMCs to reverse this phenotypic modulation depends strictly on culture conditions. Previous studies have demonstrated that applied shear stress is an important signal for vSMC phenotype. The objective of this study was to determine whether applied shear stress is capable of triggering re-differentiation of vSMCs in tissue-engineered aortas. vSMCs were isolated from ovine arteries. Cells were cultured statically or exposed to two- (2D) and three-dimensional (3D) shear stress after seeding on a tubular matrix. For 3D in vivo testing, grafts were seeded additionally with endothelial cells and implanted in the descending aorta. Particular attention was paid to the expression pattern of vSMC markers, cell ultra-structure, matrix remodeling activity, and proliferative activity. Cultured vSMCs de-differentiated during static in vitro culture, but 2D and 3D in vitro shear stress promoted re-expression of vSMC markers. During in vivo culture, vSMCs progressed toward a fully differentiated phenotype. Cells were expressing markers of differentiated vSMCs and resembled a morphologically contractile vSMC phenotype. Matrix remodeling activity and proliferative activity decreased. This study demonstrates the phenotypic plasticity of vSMCs and their ability to return to a differentiated phenotype under shear stress conditions. These results are crucial for tissue engineering of blood vessels, because they indicate for the first time the in vitro potential to regain physiological functionality of isolated vSMCs.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信