体外和体内软骨工程使用软骨细胞种子长期稳定的纤维蛋白凝胶和聚己内酯基聚氨酯支架的组合。

Daniela Eyrich, Hinrich Wiese, Gerhard Maier, Daniel Skodacek, Bernhard Appel, Hatem Sarhan, Joerg Tessmar, Rainer Staudenmaier, Magdalene M Wenzel, Achim Goepferich, Torsten Blunk
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引用次数: 128

摘要

在许多组织工程应用中,单独使用水凝胶或固体聚合物支架通常具有明显的缺点。因此,在本研究中,我们研究了长期稳定的纤维蛋白凝胶和聚氨酯支架组合在软骨工程中的潜力。原代牛软骨细胞悬浮在纤维蛋白凝胶中,随后注射到聚己内酯基聚氨酯支架中。在体外培养的4周内,细胞均匀分布在该复合系统中,并产生了大量的软骨特异性细胞外基质(ECM)成分,即糖胺聚糖(GAGs)和II型胶原。相比之下,没有纤维蛋白的细胞直接播种到支架上,导致播种效率较低,基质分布明显不均匀。植入裸鼠背部的细胞-纤维蛋白支架构建物在体内1、3、6个月后促进支架内形成足够的工程化软骨组织,其中含有均匀分布的ECM成分,如GAGs和胶原。同样,在没有注射纤维蛋白的构建体中,组织学显示不均匀,因此,与注射纤维蛋白相比,ECM分布不充分,即使在体内6个月后也是如此。引人注目的是,与预培养4周相比,体外预培养1周在体内产生了相似的结果;也就是说,预培养时间超过1周并不能促进组织发育。该复合系统被认为是工程软骨组织在整形和重建手术中临床应用的一个有希望的替代方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro and in vivo cartilage engineering using a combination of chondrocyte-seeded long-term stable fibrin gels and polycaprolactone-based polyurethane scaffolds.

The use of either a hydrogel or a solid polymeric scaffold alone is often associated with distinct drawbacks in many tissue engineering applications. Therefore, in this study, we investigated the potential of a combination of long-term stable fibrin gels and polyurethane scaffolds for cartilage engineering. Primary bovine chondrocytes were suspended in fibrin gel and subsequently injected into a polycaprolactone-based polyurethane scaffold. Cells were homogeneously distributed within this composite system and produced high amounts of cartilage-specific extracellular matrix (ECM) components, namely glycosaminoglycans (GAGs) and collagen type II, within 4 weeks of in vitro culture. In contrast, cells seeded directly onto the scaffold without fibrin resulted in a lower seeding efficiency and distinctly less homogeneous matrix distribution. Cell-fibrin-scaffold constructs implanted into the back of nude mice promoted the formation of adequate engineered cartilaginous tissue within the scaffold after 1, 3, and 6 months in vivo, containing evenly distributed ECM components, such as GAGs and collagen. Again, in constructs seeded without fibrin, histology showed an inhomogeneous and, thus, not adequate ECM distribution compared to seeding with fibrin, even after 6 months in vivo. Strikingly, a precultivation for 1 week in vitro elicited similar results in vivo compared to precultivation for 4 weeks; that is, a precultivation for longer than 1 week did not enhance tissue development. The presented composite system is suggested as a promising alternative toward clinical application of engineered cartilaginous tissue for plastic and reconstructive surgery.

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来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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