前列腺素对人脂肪组织源性间充质干细胞成骨分化的影响。

M Knippenberg, M N Helder, J M A de Blieck-Hogervorst, P I J M Wuisman, J Klein-Nulend
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引用次数: 32

摘要

脂肪组织源性间充质干细胞(AT-MSCs)目前被用于骨组织工程。进行成骨分化的AT-MSCs通过增加环氧化酶-2基因表达来响应机械负荷,环氧化酶-2是前列腺素(PG)合成的关键酶。PGs是骨中有效的多功能调节剂,对骨形成和骨吸收具有刺激和抑制作用。PGE(2),而不是PGI(2)或PGF(2),从骨髓空间招募骨祖细胞并影响其分化。我们假设PGE(2)、PGI(2)和PGF(2)可能对人AT-MSCs的成骨分化有不同的调节作用。4-14天后,PGE(2)、PGI(2)和PGF(2)(0.01-10微米)影响AT-MSCs的成骨分化,但不影响其增殖。第4天,只有PGF(2)(0.01 ~ 10微米)增加碱性磷酸酶(ALP)活性。PGE(2)(10微米)、PGI(2)(0.01-10微米)和PGF(2)(10微米)使ALP活性降低,而PGF(2)(0.1微米)使ALP活性升高。第4天,PGF(2)(0.01-0.1微米)和PGI(2)(0.01微米)上调骨桥蛋白基因表达,PGF(2)(0.01微米)上调α 1(I)前胶原基因表达。第4天PGE(2)和PGF(2)(10微米),第14天PGF(2)(1微米)下调矮子相关转录因子-2基因表达。我们得出结论,PGE(2)、PGI(2)和PGF(2)对AT-MSCs成骨分化的影响是不同的,其中PGF(2)的影响最大。因此,局部生成的PGF(2)可能最有利于促进AT-MSCs的成骨分化,从而促进骨组织工程的骨形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prostaglandins differentially affect osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.

Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are currently used for bone tissue engineering. AT-MSCs undergoing osteogenic differentiation respond to mechanical loading with increased cyclooxygenase-2 gene expression, a key enzyme in prostaglandin (PG) synthesis. PGs are potent multifunctional regulators in bone, exhibiting stimulatory and inhibitory effects on bone formation and resorption. PGE(2), but not PGI(2) or PGF(2), recruits osteoprogenitors from the bone marrow space and influences their differentiation. We hypothesize that PGE(2), PGI(2), and PGF(2) may differentially regulate osteogenic differentiation of human AT-MSCs. PGE(2), PGI(2), and PGF(2) (0.01-10 microM) affected osteogenic differentiation, but not proliferation of AT-MSCs after 4-14 days. Only PGF(2) (0.01-10 microM) increased alkaline phosphatase (ALP) activity at day 4. PGE(2) (10 microM), PGI(2) (0.01-10 microM), and PGF(2) (10 microM) decreased ALP activity, whereas PGF(2) (0.1 microM) increased ALP activity at day 14. PGF(2) (0.01-0.1 microM) and PGI(2) (0.01 microM) upregulated osteopontin gene expression, and PGF(2) (0.01 microM) upregulated alpha1(I)procollagen gene expression at day 4. PGE(2) and PGF(2) (10 microM) at day 4 and PGF(2) (1 microM) at day 14 downregulated runt-related transcription factor-2 gene expression. We conclude that PGE(2), PGI(2), and PGF(2) differentially affect osteogenic differentiation of AT-MSCs, with PGF(2) being the most potent. Thus, locally produced PGF(2) might be most beneficial in promoting osteogenic differentiation of AT-MSCs, resulting in enhanced bone formation for bone tissue engineering.

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来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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