组织工程解剖三维纤维沉积支架:新气管的设计。

Lorenzo Moroni, Maurus Curti, Manfred Welti, Stephen Korom, Walter Weder, Joost R de Wijn, Clemens A van Blitterswijk
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引用次数: 31

摘要

在人工气管结构的软骨形成方面,研究和评估了与模型设计相比,使用解剖形状支架的优势。采用三维纤维沉积(3DF)技术对支架进行快速成型。解剖支架是根据患者的计算机断层数据集制作的,并与圆柱形和环形管状支架进行比较。Lewis大鼠气管软骨细胞植入3DF支架,培养21 d。采用3-(4,5-二甲基噻唑-2基)-2,5-二苯基溴化四唑(MTT)和硫酸糖胺聚糖(GAG)测定细胞的相对数量和形成的细胞外基质(ECM)。培养3周后,通过GAG/MTT比值和扫描电镜分析,解剖支架的ECM合成明显增加,分化程度较高。有趣的是,从GAG和GAG/MTT值可以看出,支架孔隙体积和孔隙率越低,组织形成越多,细胞分化越好。支架是顺从的,在体外没有显示出任何腔管阻塞的迹象。这些结果可能会促进解剖支架作为组织再生的功能基质,不仅有助于恢复原始形状,而且还可以提高其支持更大组织形成的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anatomical 3D fiber-deposited scaffolds for tissue engineering: designing a neotrachea.

The advantage of using anatomically shaped scaffolds as compared to modeled designs was investigated and assessed in terms of cartilage formation in an artificial tracheal construct. Scaffolds were rapid prototyped with a technique named three-dimensional fiber deposition (3DF). Anatomical scaffolds were fabricated from a patient-derived computerized tomography dataset, and compared to cylindrical and toroidal tubular scaffolds. Lewis rat tracheal chondrocytes were seeded on 3DF scaffolds and cultured for 21 days. The 3-(4,5-dimethylthiazol-2yl)-2,5-dyphenyltetrazolium bromide (MTT) and sulfated glycosaminoglycan (GAG) assays were performed to measure the relative number of cells and the extracellular matrix (ECM) formed. After 3 weeks of culture, the anatomical scaffolds revealed a significant increase in ECM synthesis and a higher degree of differentiation as shown by the GAG/MTT ratio and by scanning electron microscopy analysis. Interestingly, a lower scaffold's pore volume and porosity resulted in more tissue formation and a better cell differentiation, as evidenced by GAG and GAG/MTT values. Scaffolds were compliant and did not show any signs of luminal obstruction in vitro. These results may promote anatomical scaffolds as functional matrices for tissue regeneration not only to help regain the original shape, but also for their improved capacity to support larger tissue formation.

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来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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