在小鼠组织工程室中基质诱导脂肪组织的宿主而非移植物来源。

Filip Stillaert, Michael Findlay, Jason Palmer, Rejhan Idrizi, Shirley Cheang, Aurora Messina, Keren Abberton, Wayne Morrison, Erik W Thompson
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引用次数: 93

摘要

我们最近的研究表明,在小鼠的上腹部蒂周围放置含有成纤维细胞生长因子-2 (FGF2)的基质填充腔是高度脂肪生成的。需要将这种结构与预先存在的组织或游离脂肪移植物接触。为了进一步研究新脂肪组织形成的机制,我们在严重联合免疫缺陷小鼠中植入人类脂肪活检组织和人类脂肪来源的细胞群,并在6周后使用物种特异性探针评估合成脂肪组织的来源。这些组织对人类特异性的vimentin标记呈阴性,这表明脂肪来自小鼠宿主而不是人类移植物。这得到了小鼠特异性Cot-1脱氧核糖核酸标记的强烈存在和新脂肪中缺乏人类Cot-1标记的支持。即使在不具有成熟脂肪细胞表型的细胞中,用含有培养的人间质血管部分(SVF)的FGF2/Matrigel种子的室中,只对人静脉蛋白进行强烈标记;新形成的脂肪组织未检测到人静脉球蛋白。这些发现表明,放置在腔室中的移植物具有诱导新脂肪形成的功能,而不是提供脂肪细胞前体细胞来产生新的脂肪组织,初步观察提示SVF产生诱导因子。这一惊人的发现为改进目前的脂肪组织工程方法打开了大门。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Host rather than graft origin of Matrigel-induced adipose tissue in the murine tissue-engineering chamber.

We have recently shown that Matrigel-filled chambers containing fibroblast growth factor-2 (FGF2) and placed around an epigastric pedicle in the mouse were highly adipogenic. Contact of this construct with pre-existing tissue or a free adipose graft was required. To further investigate the mechanisms underpinning formation of new adipose tissue, we seeded these chambers with human adipose biopsies and human adipose-derived cell populations in severe combined immunodeficient mice and assessed the origin of the resultant adipose tissue after 6 weeks using species-specific probes. The tissues were negative for human-specific vimentin labeling, suggesting that the fat originates from the murine host rather than the human graft. This was supported by the strong presence of mouse-specific Cot-1 deoxyribonucleic acid labeling, and the absence of human Cot-1 labeling in the new fat. Even chambers seeded with FGF2/Matrigel containing cultured human stromal-vascular fraction (SVF) labeled strongly only for human vimentin in cells that did not have a mature adipocyte phenotype; the newly formed fat tissue was negative for human vimentin. These findings indicate that grafts placed in the chamber have an inductive function for neo-adipogenesis, rather than supplying adipocyte-precursor cells to generate the new fat tissue, and preliminary observations implicate the SVF in producing inductive factors. This surprising finding opens the door for refinement of current adipose tissue-engineering approaches.

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Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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