构建胶原蛋白支架,模拟特定组织的三维结构。

Kaeuis A Faraj, Toin H van Kuppevelt, Willeke F Daamen
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引用次数: 123

摘要

每个组织和器官都有自己的三维(3D)细胞外基质(ECM)组织。用于组织工程的3D生物支架中的细胞通常根据所提供的生物支架排列新的ECM组件。因此,具有类似于特定组织的实际ECM的特定3D结构设计的支架在组织工程中可能具有很大的潜力。在这里,我们展示了,使用特定的冷冻机制,可以用胶原蛋白制造出模仿特定组织的3D结构的3D支架。给出了三个例子,即类似于肺杯状实质(肺泡)结构的支架,模拟肌腱平行胶原组织的支架,以及模拟皮肤三维组织的支架。为了制备这些组织特异性支架,我们依靠简单的技术,不需要昂贵的或定制的设备。冷冻速率、悬浮介质类型和添加剂(如乙醇)被发现是控制支架形态的主要参数。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Construction of collagen scaffolds that mimic the three-dimensional architecture of specific tissues.

Every tissue and organ has its own 3-dimensional (3D) extracellular matrix (ECM) organization. Cells in a 3D bioscaffold for tissue engineering typically align new ECM components according to the bioscaffold provided. Therefore, scaffolds with a specific 3D structural design resembling the actual ECM of a particular tissue may have great potential in tissue engineering. Here, we show that, using specific freezing regimes, 3D scaffolds that mimic the 3D architecture of specific tissues can be made from collagen. Three examples are given, namely, scaffolds resembling the cup-shaped parenchymal (alveolar) architecture of lung, scaffolds that mimic the parallel collagen organization of tendon, and scaffolds that mimic the 3D organization of skin. For the preparation of these tissue-specific scaffolds, we relied on simple techniques without the need for expensive or customized equipment. Freezing rate, type of suspension medium, and additives (e.g., ethanol) were found to be prime parameters in controlling scaffold morphology.

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Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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