小鼠关节和生长板软骨的基因表达谱。

Shintaro Yamane, Ewana Cheng, Zongbing You, A Hari Reddi
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引用次数: 44

摘要

关节软骨难以修复和再生。组织工程和再生医学是治疗关节软骨损伤的潜在策略。深入了解关节软骨和生长板软骨细胞的基因表达谱将是软骨组织工程的重要前提。再生是胚胎发育和形态发生的再现。采用激光捕获显微解剖方法,对14日龄C57BL/6J小鼠关节表面软骨细胞和生长板静息区软骨细胞进行了捕获。总RNA被单独纯化,汇集,并通过两轮体外转录扩增。标记的cRNA探针使用Affymetrix基因芯片小鼠基因组430 2.0阵列进行分析。我们发现107个基因在关节表面软骨细胞中高表达,130个基因在生长板静息区软骨细胞中高表达(>或= 5倍)。主要基质蛋白聚集蛋白和II型胶原表达相似,而关节表面软骨细胞和生长板静息区软骨细胞表达的形态因子和生长因子存在差异。本研究的结果将用于组织工程软骨的评价。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gene expression profiling of mouse articular and growth plate cartilage.

Articular cartilage is recalcitrant to repair and regeneration. Tissue engineering and regenerative medicine are potential strategies to treat the damage to articular cartilage. A thorough understanding of the gene expression profiles in articular cartilage and growth plate chondrocytes will be an important prerequisite for tissue engineering of cartilage. Regeneration is a recapitulation of embryonic development and morphogenesis. We used laser capture microdissection to capture the surface articular chondrocytes and the resting zone chondrocytes of growth plate from 14-day-old C57BL/6J mice. Total RNA was individually purified, pooled, and amplified by two rounds of in vitro transcription. Labeled cRNA probes were analyzed using the Affymetrix GeneChip Mouse Genome 430 2.0 Array. We identified 107 genes that were highly expressed by the surface articular chondrocytes and 130 genes that were highly expressed by the resting zone chondrocytes of growth plate (> or = fivefold). The expression of major matrix proteins aggrecan and collagen II were similar, while several morphogens and growth factors were differentially expressed by the surface articular chondrocytes and the resting zone chondrocytes of growth plate. The results of this investigation will be of use in the evaluation of tissue engineered cartilage.

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Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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