缺氧在体外组织工程中的作用。

Jos Malda, Travis J Klein, Zee Upton
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引用次数: 277

摘要

氧是体内细胞功能和伤口修复的有效调节剂。缺氧可以创造一个潜在的致命环境,限制细胞呼吸和生长,或者,通过缺氧诱导因子-1途径,增强特定细胞外基质成分的产生和增加血管生成。为了在体外产生临床相关的组织工程移植物,应该解决这些缺氧的不同作用。通过培养基和组织的扩散通常限制体外氧运输,导致缺氧区域和限制活组织厚度。克服转运限制的方法包括生物反应器培养、人工微血管支架、氧气载体和高压氧舱。作为一种替代方法,植入后血管生成可以通过将内皮细胞、基因修饰细胞或特定因子(包括血管内皮生长因子)加入支架或在植入前将移植物暴露于缺氧环境中来增强。更好地了解缺氧的作用将有助于预防常见问题,并在工程组织中利用缺氧的潜在益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The roles of hypoxia in the in vitro engineering of tissues.

Oxygen is a potent modulator of cell function and wound repair in vivo. The lack of oxygen (hypoxia) can create a potentially lethal environment and limit cellular respiration and growth or, alternatively, enhance the production of the specific extracellular matrix components and increase angiogenesis through the hypoxia-inducible factor-1 pathway. For the in vitro generation of clinically relevant tissue-engineered grafts, these divergent actions of hypoxia should be addressed. Diffusion through culture medium and tissue typically limits oxygen transport in vitro, leading to hypoxic regions and limiting the viable tissue thickness. Approaches to overcoming the transport limitations include culture with bioreactors, scaffolds with artificial microvasculature, oxygen carriers, and hyperbaric oxygen chambers. As an alternate approach, angiogenesis after implantation may be enhanced by incorporating endothelial cells, genetically modified cells, or specific factors (including vascular endothelial growth factor) into the scaffold or exposing the graft to a hypoxic environment just before implantation. Better understanding of the roles of hypoxia will help prevent common problems and exploit potential benefits of hypoxia in engineered tissues.

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来源期刊
Tissue engineering
Tissue engineering CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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