Effects of growth factors on testicular morphogenesis.

Since the discovery of the sex-determining gene Sry in 1990, research effort has focused on the events downstream of its expression. A range of different experimental approaches including gene expression, knocking-out and knocking-in genes of interest, and cell and tissue culture techniques have been applied, highlighting the importance of growth factors at all stages of testicular morphogenesis. Migration of primordial germ cells and the mesonephric precursors of peritubular myoid cells and endothelial cells to the gonad is under growth factor control. Proliferation of both germ cells and somatic cells within the gonadal primordium is also controlled by cytokines as is the interaction of Sertoli cells (with each other and with the extracellular matrix) to form testicular cords. Several growth factors/growth factor families (e.g., platelet-derived growth factor, fibroblast growth factor family, TGFbeta family, and neurotrophins) have emerged as key players, exerting an influence at different time points and steps in organogenesis. Although most evidence has emerged in the mouse, comparative studies are important in elucidating the variety, potential, and evolution of control mechanisms.