人类AP3B2新剪接变体AP3B2_v2的特征

Chunjing Chen, Xianqiong Zou, Chaoneng Ji, Shuo Zhao, Lei Lv, Shaohua Gu, Yi Xie, Yumin Mao
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引用次数: 1

摘要

通过对人胎脑cDNA文库的大规模测序分析,分离到一种新的人类AP3B2剪接变体AP3B2_v2。AP3B2_v2 cDNA全长1171 bp。序列分析显示,AP3B2_v2缺失了AP3B2_v1中存在的22个外显子,导致AP3B2_v2可能是一个不同的蛋白。推导出的蛋白长度分别为145个氨基酸(命名为AP3B2_v2)和1082个氨基酸(命名为AP3B2_v1),共享c端145个氨基酸。RT-PCR分析显示,AP3B2_v2基因在多种成人组织中均有表达。AP3B2_v2在脑和睾丸的表达水平较高。而在肾脏、胰腺、脾脏、胸腺、前列腺、卵巢和小肠中均检测到低水平表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of AP3B2_v2, a novel splice variant of human AP3B2.

A novel splice variant of human AP3B2, named AP3B2_v2, was isolated through the large-scale sequencing analysis of a human fetal brain cDNA library. The AP3B2_v2 cDNA is 1171 bp in length. Sequence analysis revealed AP3B2_v2 missed 22 exons that existed in AP3B2_v1, leading to a different putative protein. The deduced proteins were 145 amino acids (designated as AP3B2_v2) and 1082 amino acids (AP3B2_v1) in length, sharing the C-terminal 145 amino acids. RT-PCR analysis showed that human AP3B2_v2 were expressed in several human adult tissues analyzed. The expression levels of AP3B2_v2 were relatively high in brain and testis. In contrast, low levels of expression were detected in kidney, pancreas, spleen, thymus, prostate, ovary and small intestine.

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