Chunjing Chen, Xianqiong Zou, Chaoneng Ji, Shuo Zhao, Lei Lv, Shaohua Gu, Yi Xie, Yumin Mao
{"title":"人类AP3B2新剪接变体AP3B2_v2的特征","authors":"Chunjing Chen, Xianqiong Zou, Chaoneng Ji, Shuo Zhao, Lei Lv, Shaohua Gu, Yi Xie, Yumin Mao","doi":"10.1080/10425170600842121","DOIUrl":null,"url":null,"abstract":"<p><p>A novel splice variant of human AP3B2, named AP3B2_v2, was isolated through the large-scale sequencing analysis of a human fetal brain cDNA library. The AP3B2_v2 cDNA is 1171 bp in length. Sequence analysis revealed AP3B2_v2 missed 22 exons that existed in AP3B2_v1, leading to a different putative protein. The deduced proteins were 145 amino acids (designated as AP3B2_v2) and 1082 amino acids (AP3B2_v1) in length, sharing the C-terminal 145 amino acids. RT-PCR analysis showed that human AP3B2_v2 were expressed in several human adult tissues analyzed. The expression levels of AP3B2_v2 were relatively high in brain and testis. In contrast, low levels of expression were detected in kidney, pancreas, spleen, thymus, prostate, ovary and small intestine.</p>","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 3","pages":"165-8"},"PeriodicalIF":0.0000,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170600842121","citationCount":"1","resultStr":"{\"title\":\"Characterization of AP3B2_v2, a novel splice variant of human AP3B2.\",\"authors\":\"Chunjing Chen, Xianqiong Zou, Chaoneng Ji, Shuo Zhao, Lei Lv, Shaohua Gu, Yi Xie, Yumin Mao\",\"doi\":\"10.1080/10425170600842121\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A novel splice variant of human AP3B2, named AP3B2_v2, was isolated through the large-scale sequencing analysis of a human fetal brain cDNA library. The AP3B2_v2 cDNA is 1171 bp in length. Sequence analysis revealed AP3B2_v2 missed 22 exons that existed in AP3B2_v1, leading to a different putative protein. The deduced proteins were 145 amino acids (designated as AP3B2_v2) and 1082 amino acids (AP3B2_v1) in length, sharing the C-terminal 145 amino acids. RT-PCR analysis showed that human AP3B2_v2 were expressed in several human adult tissues analyzed. The expression levels of AP3B2_v2 were relatively high in brain and testis. In contrast, low levels of expression were detected in kidney, pancreas, spleen, thymus, prostate, ovary and small intestine.</p>\",\"PeriodicalId\":11197,\"journal\":{\"name\":\"DNA sequence : the journal of DNA sequencing and mapping\",\"volume\":\"18 3\",\"pages\":\"165-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/10425170600842121\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"DNA sequence : the journal of DNA sequencing and mapping\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10425170600842121\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA sequence : the journal of DNA sequencing and mapping","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10425170600842121","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Characterization of AP3B2_v2, a novel splice variant of human AP3B2.
A novel splice variant of human AP3B2, named AP3B2_v2, was isolated through the large-scale sequencing analysis of a human fetal brain cDNA library. The AP3B2_v2 cDNA is 1171 bp in length. Sequence analysis revealed AP3B2_v2 missed 22 exons that existed in AP3B2_v1, leading to a different putative protein. The deduced proteins were 145 amino acids (designated as AP3B2_v2) and 1082 amino acids (AP3B2_v1) in length, sharing the C-terminal 145 amino acids. RT-PCR analysis showed that human AP3B2_v2 were expressed in several human adult tissues analyzed. The expression levels of AP3B2_v2 were relatively high in brain and testis. In contrast, low levels of expression were detected in kidney, pancreas, spleen, thymus, prostate, ovary and small intestine.