整合素样蛋白和α -微管蛋白在玉米根细胞中的共定位及其可能的相互作用。

植物生理与分子生物学学报 Pub Date : 2007-04-01
Bing Lv, Feng Chen, Zhong-Hua Gong, Jian-Sheng Liang
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引用次数: 0

摘要

植物细胞壁-质膜-细胞骨架(CW-PM-CSK)连续体在植物对环境信号的响应调控中起着重要作用。我们在前期研究中发现,拟南芥和玉米的质膜中存在一种整合素样蛋白,介导细胞壁与质膜的相互作用以及细胞对渗透胁迫的反应。本研究采用间接免疫荧光技术结合药理学方法,研究了整合素样蛋白和α -微管蛋白在玉米根细胞中的共定位及其可能的相互作用。与对照相比,经GRGDS处理后,合成的含有特异结合整合素样蛋白的RGD结构域的肽段,整合素样蛋白在质膜上的分布更加均匀,α -微管蛋白阵列的密度降低。然而,用不含RGD结构域的GRGDS类似物SDGRG处理对它们没有影响。微管解聚或稳定试剂对质膜上的分布或整合素样蛋白的表达均有影响。上述结果表明,整合素样蛋白在质膜上的分布与微管阵列的排列密切相关。整合素样蛋白介导细胞壁和质膜之间的相互作用,这种相互作用反过来影响微管阵列的排列。细胞骨架动力学的变化对整合素样蛋白在质膜上的分布有显著影响。这些结果有力地说明了植物细胞中整合素样蛋白与细胞骨架之间存在着非常复杂的相互作用。植物对细胞内和细胞外信号的响应机制值得深入研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The co-localization and possible interactions of integrin-like protein and alpha-tubulin in the root cells of Zea mays.

Plant cell wall-plasma membrane-cytoskeleton (CW-PM-CSK) continuum plays important roles in the regulation of plant responses to environmental cues. It has been found in our previous study that an integrin-like protein identified in Arabidopsis and Zea mays plasma membrane mediates the interactions between cell wall and plasma membrane and cell responses to osmotic stress. In the present study, the co-localization of integrin-like protein and alpha-tubulin and their possible interactions were investigated in the root cells of maize using an indirect immunofluorescence technique combined with pharmacological approach. As compared with the control, after treatment with GRGDS, a synthetic peptide contained the RGD domain which binds specifically to integrin-like protein, the integrin-like protein was distributed more evenly over the plasma membrane and the density of alpha-tubulin array became lower. However, treatment with SDGRG, a GRGDS analog which does not contain the RGD domain, had no influences on them. Microtubule-depolymering or -stabilizing reagents exerted their effects either on the distribution over the plasma membrane or on the expression of integrin-like protein. The above results showed that the distribution of integrin-like protein on plasma membrane was closely linked to the alignment of microtubule arrays. Integrin-like protein mediates the interaction between cell wall and plasma membrane, and this interaction in return affected the alignment of microtubule arrays. Changes in cytoskeleton dynamics had significant effect on the distribution of integrin-like protein on the plasma membrane. These results strongly suggest that there are interactions between integrin-like protein and cytoskeleton in plant cells which are very complicated. It is worthy of extensive study to work out the mechanisms of plant responses to intracellular and extracellular signals.

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