壁虎附睾和输精管的超微结构研究及其腔液蛋白的电泳图谱。

I Aranha, M Bhagya, H N Yajurvedi
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引用次数: 0

摘要

附睾和输精管的光镜、组织化学和透射电镜研究显示,这些器官腔内的上皮细胞中存在PAS阳性分泌颗粒。研究了马布亚龙(Mabuya carinata)生殖周期繁殖期和非繁殖期睾丸、附睾三个部位和输精管腔液蛋白的一维SDS凝胶电泳图谱和总蛋白含量。在繁殖季节,在睾丸管腔液中发现25个蛋白质条带,在附睾前管腔液中发现26个,在附睾中后管腔液中发现28个。在附睾前部出现10条新的蛋白带,而在附睾中部出现5条新的蛋白带,说明附睾蛋白分泌的区域差异。与睾丸和附睾相比,输精管腔液蛋白条带数最多(32条),总蛋白含量最高(9.07 mg/ml)。输精管内出现4条新的蛋白带。非繁殖期睾丸、附睾和输精管管腔液中蛋白带数量明显少于繁殖期。与蛋白带数量减少一致的是,在非繁殖期,所有组织样品的总蛋白浓度都显著降低。研究结果表明,海蛸雄性生殖道管腔液中蛋白质分泌数量和蛋白质含量存在季节性差异。这是在爬行动物中首次通过对睾丸、附睾和输精管管腔内容物同时进行电泳分析,揭示附睾和输精管中新蛋白的出现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultrastructural study of the epididymis and the vas deferens and electrophoretic profile of their luminal fluid proteins in the lizard Mabuya carinata.

The light microscopy, histochemical and TEM studies of the epididymis and the vas deferens revealed the presence of PAS positive secretory granules in the epithelial cells lining the lumen of these organs. One dimensional SDS gel electrophoretic pattern of luminal fluid proteins and the total protein content of the testis, three regions of the epididymis and the vas deferens of the lizard, Mabuya carinata were studied during breeding and nonbreeding season of the reproductive cycle. During breeding season, 25 protein bands in the testicular luminal fluid, 26 in the anterior epididymal luminal fluid and 28 in the middle and posterior epididymal luminal fluid were found. Ten new protein bands appeared in the anterior epididymal region whereas five new protein bands appeared in the middle region of the epididymis indicating regional difference in protein secretions of the epididymis. Vas deferens luminal fluid showed the highest number of protein bands (32) and the highest total protein content (9.07 mg/ml) compared to the testis and the epididymis. Four new protein bands appeared in the vas deferens. Number of protein bands in the luminal fluids of testis, epididymis and the vas deferens were significantly reduced during nonbreeding season compared to those of the breeding season. Consistent with the decrease in the number of protein bands, there was a significant reduction in the total protein concentration in all the tissue samples during nonbreeding season. The results indicate seasonal differences in number of proteins secreted and quantity of proteins in the luminal fluid of male reproductive tract of M. carinata. This is the first study in reptiles revealing appearance of new proteins in epididymis, and vas deferens by conducting simultaneous electrophoretic profile of testicular, epididymal and vas deferens luminal contents.

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