S M Mostafa Kamal Khan, Chisato Yoshimura, Mikihiko Arikawa, Gen Omura, Satoru Nishiyama, Yasutaka Suetomo, Soichiro Kakuta, Toshinobu Suzaki
{"title":"收缩蛇尾虫轴足降解:一种检测水生环境中重金属毒性的新型生物测定系统。","authors":"S M Mostafa Kamal Khan, Chisato Yoshimura, Mikihiko Arikawa, Gen Omura, Satoru Nishiyama, Yasutaka Suetomo, Soichiro Kakuta, Toshinobu Suzaki","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>We observed the physiological effects of zinc, lead, mercury, copper, cadmium, and arsenic on the axopodia of the centrohelid heliozoon Raphidiophrys contractilis. In the presence of these heavy metal ions, the axopodial length of the heliozoon decreased in a concentration-dependent manner. When the heavy metal ions were examined at the same concentration, mercury produced the strongest effect on axopodia. At a high concentration (> 10-3 M) of any of the heavy metal ions examined, axopodia disappeared and cells became disrupted. Axopodia were also degraded by the addition of solutions with an acidic (< or = 6) or basic (> or = 8) pH. These observations indicate the toxic effects of heavy metal ions and non-neutral pHs on axopodial length, and also signify that R. contractilis can be used as an effective biological tool for the study of metal poisoning in eukaryotic cells.</p>","PeriodicalId":87178,"journal":{"name":"Environmental sciences : an international journal of environmental physiology and toxicology","volume":"13 4","pages":"193-200"},"PeriodicalIF":0.0000,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Axopodial degradation in the heliozoon Raphidiophrys contractilis: a novel bioassay system for detecting heavy metal toxicity in an aquatic environment.\",\"authors\":\"S M Mostafa Kamal Khan, Chisato Yoshimura, Mikihiko Arikawa, Gen Omura, Satoru Nishiyama, Yasutaka Suetomo, Soichiro Kakuta, Toshinobu Suzaki\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We observed the physiological effects of zinc, lead, mercury, copper, cadmium, and arsenic on the axopodia of the centrohelid heliozoon Raphidiophrys contractilis. In the presence of these heavy metal ions, the axopodial length of the heliozoon decreased in a concentration-dependent manner. When the heavy metal ions were examined at the same concentration, mercury produced the strongest effect on axopodia. At a high concentration (> 10-3 M) of any of the heavy metal ions examined, axopodia disappeared and cells became disrupted. Axopodia were also degraded by the addition of solutions with an acidic (< or = 6) or basic (> or = 8) pH. These observations indicate the toxic effects of heavy metal ions and non-neutral pHs on axopodial length, and also signify that R. contractilis can be used as an effective biological tool for the study of metal poisoning in eukaryotic cells.</p>\",\"PeriodicalId\":87178,\"journal\":{\"name\":\"Environmental sciences : an international journal of environmental physiology and toxicology\",\"volume\":\"13 4\",\"pages\":\"193-200\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental sciences : an international journal of environmental physiology and toxicology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental sciences : an international journal of environmental physiology and toxicology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Axopodial degradation in the heliozoon Raphidiophrys contractilis: a novel bioassay system for detecting heavy metal toxicity in an aquatic environment.
We observed the physiological effects of zinc, lead, mercury, copper, cadmium, and arsenic on the axopodia of the centrohelid heliozoon Raphidiophrys contractilis. In the presence of these heavy metal ions, the axopodial length of the heliozoon decreased in a concentration-dependent manner. When the heavy metal ions were examined at the same concentration, mercury produced the strongest effect on axopodia. At a high concentration (> 10-3 M) of any of the heavy metal ions examined, axopodia disappeared and cells became disrupted. Axopodia were also degraded by the addition of solutions with an acidic (< or = 6) or basic (> or = 8) pH. These observations indicate the toxic effects of heavy metal ions and non-neutral pHs on axopodial length, and also signify that R. contractilis can be used as an effective biological tool for the study of metal poisoning in eukaryotic cells.