{"title":"[角质细胞生长因子对Hep-2细胞系胞外信号调节激酶表达和活性的影响]。","authors":"Rong Fang, Jin Hu, Hongtao Zhen, Yonghua Cui","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To observe effects of keratinocyte growth factor (KGF) on the proliferation of Hep-2 cell line; the activity and expression of extracellular signal-regulated kinase (ERK).</p><p><strong>Method: </strong>Cell line proliferation was measured by Flow cytometry and MTT assay; ERK activity was measured by ERKl/2 immuno-precipitation kinase assay kit; and p ERK expression was assessed by Western blot.</p><p><strong>Result: </strong>KGF can improve the proliferation of Hep-2 cell line. The intracellular ERK activity and p-ERK expression were increased by different concentrations of KGF.</p><p><strong>Conclusion: </strong>KGF can stimulated proliferation of Hep-2 cell line with enhancing activity of ERK and expression of p-ERK.</p>","PeriodicalId":79680,"journal":{"name":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","volume":"20 24","pages":"1132-4"},"PeriodicalIF":0.0000,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effects of keratinocyte growth factor on the expression and activity of extracellular signal-regulated kinase in Hep-2 cell line].\",\"authors\":\"Rong Fang, Jin Hu, Hongtao Zhen, Yonghua Cui\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To observe effects of keratinocyte growth factor (KGF) on the proliferation of Hep-2 cell line; the activity and expression of extracellular signal-regulated kinase (ERK).</p><p><strong>Method: </strong>Cell line proliferation was measured by Flow cytometry and MTT assay; ERK activity was measured by ERKl/2 immuno-precipitation kinase assay kit; and p ERK expression was assessed by Western blot.</p><p><strong>Result: </strong>KGF can improve the proliferation of Hep-2 cell line. The intracellular ERK activity and p-ERK expression were increased by different concentrations of KGF.</p><p><strong>Conclusion: </strong>KGF can stimulated proliferation of Hep-2 cell line with enhancing activity of ERK and expression of p-ERK.</p>\",\"PeriodicalId\":79680,\"journal\":{\"name\":\"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology\",\"volume\":\"20 24\",\"pages\":\"1132-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Effects of keratinocyte growth factor on the expression and activity of extracellular signal-regulated kinase in Hep-2 cell line].
Objective: To observe effects of keratinocyte growth factor (KGF) on the proliferation of Hep-2 cell line; the activity and expression of extracellular signal-regulated kinase (ERK).
Method: Cell line proliferation was measured by Flow cytometry and MTT assay; ERK activity was measured by ERKl/2 immuno-precipitation kinase assay kit; and p ERK expression was assessed by Western blot.
Result: KGF can improve the proliferation of Hep-2 cell line. The intracellular ERK activity and p-ERK expression were increased by different concentrations of KGF.
Conclusion: KGF can stimulated proliferation of Hep-2 cell line with enhancing activity of ERK and expression of p-ERK.
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