Christianne Anne Rachel Lok , Anita N. Böing , Pieter H. Reitsma , Joris A.M. van der Post , Ed van Bavel , Kees Boer , Augueste Sturk , Rienk Nieuwland
{"title":"内皮细胞中炎症相关基因的表达不受子痫前期患者微颗粒的直接影响","authors":"Christianne Anne Rachel Lok , Anita N. Böing , Pieter H. Reitsma , Joris A.M. van der Post , Ed van Bavel , Kees Boer , Augueste Sturk , Rienk Nieuwland","doi":"10.1016/j.lab.2006.02.004","DOIUrl":null,"url":null,"abstract":"<div><p>Background: Inflammation and endothelial dysfunction are prominent in preeclampsia. Microparticles (MPs) may link these processes, as MPs induce the production of pro-inflammatory cytokines by endothelial cells and cause endothelial dysfunction. Aim: To study changes in expression of inflammation-related genes in human endothelial cells in response to MPs from preeclamptic patients. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated for various time intervals in the absence or presence of isolated MP fractions from preeclamptic patients (n = 3), normotensive pregnant women (n = 3), non-pregnant controls (n = 3), and interleukin (IL)-1α as a positive control. Total RNA was isolated and used for multiplex ligation-dependent probe amplification (MLPA) and real-time polymerase chain reaction (PCR). Results: IL-1α enhanced the expression of IL-1α, IL-2, IL-6, and IL-8; nuclear factor of kappa light chain enhancer in B-cells (NFκB)-1, NFκB-2, and NFκB-inhibitor; cyclin-dependent kinase inhibitor and monocyte chemotactic protein-1; and transiently increased tissue factor expression. RNA expression of inflammation-related genes and genes encoding adhesion receptors, however, were unaffected by any of the MP fractions tested. Conclusion: MLPA is a suitable assay to test the inflammatory status of endothelial cells, because incubation with IL-1α triggered substantial changes in RNA expression in endothelial cells. Taken together, it seems unlikely that MPs from preeclamptic patients induce endothelial dysfunction by directly affecting the expression of inflammation-related genes in these cells.</p></div>","PeriodicalId":16273,"journal":{"name":"Journal of Laboratory and Clinical Medicine","volume":"147 6","pages":"Pages 310-320"},"PeriodicalIF":0.0000,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.lab.2006.02.004","citationCount":"29","resultStr":"{\"title\":\"Expression of inflammation-related genes in endothelial cells is not directly affected by microparticles from preeclamptic patients\",\"authors\":\"Christianne Anne Rachel Lok , Anita N. Böing , Pieter H. Reitsma , Joris A.M. van der Post , Ed van Bavel , Kees Boer , Augueste Sturk , Rienk Nieuwland\",\"doi\":\"10.1016/j.lab.2006.02.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Background: Inflammation and endothelial dysfunction are prominent in preeclampsia. Microparticles (MPs) may link these processes, as MPs induce the production of pro-inflammatory cytokines by endothelial cells and cause endothelial dysfunction. Aim: To study changes in expression of inflammation-related genes in human endothelial cells in response to MPs from preeclamptic patients. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated for various time intervals in the absence or presence of isolated MP fractions from preeclamptic patients (n = 3), normotensive pregnant women (n = 3), non-pregnant controls (n = 3), and interleukin (IL)-1α as a positive control. Total RNA was isolated and used for multiplex ligation-dependent probe amplification (MLPA) and real-time polymerase chain reaction (PCR). Results: IL-1α enhanced the expression of IL-1α, IL-2, IL-6, and IL-8; nuclear factor of kappa light chain enhancer in B-cells (NFκB)-1, NFκB-2, and NFκB-inhibitor; cyclin-dependent kinase inhibitor and monocyte chemotactic protein-1; and transiently increased tissue factor expression. RNA expression of inflammation-related genes and genes encoding adhesion receptors, however, were unaffected by any of the MP fractions tested. Conclusion: MLPA is a suitable assay to test the inflammatory status of endothelial cells, because incubation with IL-1α triggered substantial changes in RNA expression in endothelial cells. Taken together, it seems unlikely that MPs from preeclamptic patients induce endothelial dysfunction by directly affecting the expression of inflammation-related genes in these cells.</p></div>\",\"PeriodicalId\":16273,\"journal\":{\"name\":\"Journal of Laboratory and Clinical Medicine\",\"volume\":\"147 6\",\"pages\":\"Pages 310-320\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.lab.2006.02.004\",\"citationCount\":\"29\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Laboratory and Clinical Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022214306001065\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Laboratory and Clinical Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022214306001065","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Expression of inflammation-related genes in endothelial cells is not directly affected by microparticles from preeclamptic patients
Background: Inflammation and endothelial dysfunction are prominent in preeclampsia. Microparticles (MPs) may link these processes, as MPs induce the production of pro-inflammatory cytokines by endothelial cells and cause endothelial dysfunction. Aim: To study changes in expression of inflammation-related genes in human endothelial cells in response to MPs from preeclamptic patients. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated for various time intervals in the absence or presence of isolated MP fractions from preeclamptic patients (n = 3), normotensive pregnant women (n = 3), non-pregnant controls (n = 3), and interleukin (IL)-1α as a positive control. Total RNA was isolated and used for multiplex ligation-dependent probe amplification (MLPA) and real-time polymerase chain reaction (PCR). Results: IL-1α enhanced the expression of IL-1α, IL-2, IL-6, and IL-8; nuclear factor of kappa light chain enhancer in B-cells (NFκB)-1, NFκB-2, and NFκB-inhibitor; cyclin-dependent kinase inhibitor and monocyte chemotactic protein-1; and transiently increased tissue factor expression. RNA expression of inflammation-related genes and genes encoding adhesion receptors, however, were unaffected by any of the MP fractions tested. Conclusion: MLPA is a suitable assay to test the inflammatory status of endothelial cells, because incubation with IL-1α triggered substantial changes in RNA expression in endothelial cells. Taken together, it seems unlikely that MPs from preeclamptic patients induce endothelial dysfunction by directly affecting the expression of inflammation-related genes in these cells.