Kelly M Johnson, Xavier Alvarez, Olga N Borkhsenious, H Michael Kubisch
{"title":"体外培养的牛囊胚中干扰素-tau的细胞核和细胞质定位。","authors":"Kelly M Johnson, Xavier Alvarez, Olga N Borkhsenious, H Michael Kubisch","doi":"10.1051/rnd:2005060","DOIUrl":null,"url":null,"abstract":"<p><p>Experiments were conducted to detect interferon-tau in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN-tau in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN-tau in the trophectoderm of blastocysts. The distribution of IFN-tau appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 1","pages":"97-104"},"PeriodicalIF":0.0000,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005060","citationCount":"13","resultStr":"{\"title\":\"Nuclear and cytoplasmic localization of interferon-tau in in vitro-produced bovine blastocysts.\",\"authors\":\"Kelly M Johnson, Xavier Alvarez, Olga N Borkhsenious, H Michael Kubisch\",\"doi\":\"10.1051/rnd:2005060\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Experiments were conducted to detect interferon-tau in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN-tau in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN-tau in the trophectoderm of blastocysts. The distribution of IFN-tau appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.</p>\",\"PeriodicalId\":21133,\"journal\":{\"name\":\"Reproduction, nutrition, development\",\"volume\":\"46 1\",\"pages\":\"97-104\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1051/rnd:2005060\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproduction, nutrition, development\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1051/rnd:2005060\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction, nutrition, development","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1051/rnd:2005060","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Nuclear and cytoplasmic localization of interferon-tau in in vitro-produced bovine blastocysts.
Experiments were conducted to detect interferon-tau in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN-tau in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN-tau in the trophectoderm of blastocysts. The distribution of IFN-tau appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.