Anthoula Chatzikyriakidou, Christos Yapijakis, Nikolaos Sofikitis, Dimitrios Vassilopoulos, Ioannis Georgiou
{"title":"雄激素受体基因三核苷酸重复等位基因扩增的实时PCR分析。","authors":"Anthoula Chatzikyriakidou, Christos Yapijakis, Nikolaos Sofikitis, Dimitrios Vassilopoulos, Ioannis Georgiou","doi":"10.1007/BF03260095","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The expansion of specific trinucleotide repeats results in certain genetic disorders.</p><p><strong>Method: </strong>Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene.</p><p><strong>Result: </strong>The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C.</p><p><strong>Conclusion: </strong>Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.</p>","PeriodicalId":79690,"journal":{"name":"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology","volume":"9 4","pages":"217-9"},"PeriodicalIF":0.0000,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF03260095","citationCount":"2","resultStr":"{\"title\":\"Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene.\",\"authors\":\"Anthoula Chatzikyriakidou, Christos Yapijakis, Nikolaos Sofikitis, Dimitrios Vassilopoulos, Ioannis Georgiou\",\"doi\":\"10.1007/BF03260095\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The expansion of specific trinucleotide repeats results in certain genetic disorders.</p><p><strong>Method: </strong>Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene.</p><p><strong>Result: </strong>The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C.</p><p><strong>Conclusion: </strong>Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.</p>\",\"PeriodicalId\":79690,\"journal\":{\"name\":\"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology\",\"volume\":\"9 4\",\"pages\":\"217-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/BF03260095\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/BF03260095\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF03260095","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene.
Introduction: The expansion of specific trinucleotide repeats results in certain genetic disorders.
Method: Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene.
Result: The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C.
Conclusion: Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
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