{"title":"用PCR和序列分析方法测定4个毒力基因在空肠弯曲杆菌中的流行率。","authors":"Vasilios Kordinas, Chryssoula Nicolaou, Anastassios Ioannidis, Eleni Papavasileiou, Nicolaos John Legakis, Stylianos Chatzipanagiotou","doi":"10.1007/BF03260094","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The presence of four virulence genes (racR, wlaN, cgtB, virB11) in 356 Campylobacter jejuni strains isolated from confirmed clinical cases was examined by PCR and sequence analysis. The investigated genes were chosen on the basis of their variation in prevalence.</p><p><strong>Methods: </strong>The virulence genes were detected by PCR and the amplified products were submitted for sequence analysis.</p><p><strong>Results: </strong>The gene with the highest prevalence was racR (87.08%). virB was present in only 1.69% of the C. jejuni strains, and wlaN and cgtB were detected in 16.01% and 24.44%, respectively. Five strains associated with Guillain-Barré syndrome and Miller-Fischer syndrome out of the total of 356 (1.40%) were positive for cgtB.</p><p><strong>Conclusion: </strong>Our findings suggest that racR may encode factors necessary for bacterial pathogenicity in humans, while the roles of the other three genes remain ambiguous.</p>","PeriodicalId":79690,"journal":{"name":"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology","volume":"9 4","pages":"211-5"},"PeriodicalIF":0.0000,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF03260094","citationCount":"8","resultStr":"{\"title\":\"Prevalence of four virulence genes in Campylobacter jejuni determined by PCR and sequence analysis.\",\"authors\":\"Vasilios Kordinas, Chryssoula Nicolaou, Anastassios Ioannidis, Eleni Papavasileiou, Nicolaos John Legakis, Stylianos Chatzipanagiotou\",\"doi\":\"10.1007/BF03260094\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The presence of four virulence genes (racR, wlaN, cgtB, virB11) in 356 Campylobacter jejuni strains isolated from confirmed clinical cases was examined by PCR and sequence analysis. The investigated genes were chosen on the basis of their variation in prevalence.</p><p><strong>Methods: </strong>The virulence genes were detected by PCR and the amplified products were submitted for sequence analysis.</p><p><strong>Results: </strong>The gene with the highest prevalence was racR (87.08%). virB was present in only 1.69% of the C. jejuni strains, and wlaN and cgtB were detected in 16.01% and 24.44%, respectively. Five strains associated with Guillain-Barré syndrome and Miller-Fischer syndrome out of the total of 356 (1.40%) were positive for cgtB.</p><p><strong>Conclusion: </strong>Our findings suggest that racR may encode factors necessary for bacterial pathogenicity in humans, while the roles of the other three genes remain ambiguous.</p>\",\"PeriodicalId\":79690,\"journal\":{\"name\":\"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology\",\"volume\":\"9 4\",\"pages\":\"211-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/BF03260094\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/BF03260094\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF03260094","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Prevalence of four virulence genes in Campylobacter jejuni determined by PCR and sequence analysis.
Introduction: The presence of four virulence genes (racR, wlaN, cgtB, virB11) in 356 Campylobacter jejuni strains isolated from confirmed clinical cases was examined by PCR and sequence analysis. The investigated genes were chosen on the basis of their variation in prevalence.
Methods: The virulence genes were detected by PCR and the amplified products were submitted for sequence analysis.
Results: The gene with the highest prevalence was racR (87.08%). virB was present in only 1.69% of the C. jejuni strains, and wlaN and cgtB were detected in 16.01% and 24.44%, respectively. Five strains associated with Guillain-Barré syndrome and Miller-Fischer syndrome out of the total of 356 (1.40%) were positive for cgtB.
Conclusion: Our findings suggest that racR may encode factors necessary for bacterial pathogenicity in humans, while the roles of the other three genes remain ambiguous.
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