[与水稻早期稳定性相关的SSR标记]。

Li-Jun Zhou, Guang-Hui Ao, Xian-Jun Wu, Shi-Gui Li
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引用次数: 0

摘要

由早期稳定的9个水稻品系与7个品种(Oryza sativa L.)杂交而成的130个杂交种。在F2群体中,观察到32个具有不同农艺性状的均匀品系。在这些均匀菌株的相同组合中,有菌株以孟德尔方式分离。SSR标记分析表明,均匀菌株的F2和F3群体及其F1植株显示出相同的标记,表明所有均匀菌株都是纯合子。亲本的所有标记带同时出现在其后代染色体上,表明这一均匀菌株一定是真正的杂交起源。分离株系F2群体及其F1植株的SSR标记表明,分离株系具有杂合性。在大多数均匀品系中,RM276、RM258、RM248和RM1位点的SSR标记频带位点出现与亲本不同的高频频带位点。我们认为,在配子形成过程中,ga-1、Rf和ga-8相关基因以一定频率突变,随后受精卵发生体细胞减数分裂。因此单倍体细胞可以发育成纯合子胚,F1植株在早期是纯合子的,后代稳定。
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[SSR markers linked with early stability in rice].

One hundred and thirty hybrids derived from the crosses between nine rice strains stabilized in early generation and seven cultivars (Oryza sativa L.). In their F2 populations, 32 uniform strains of different agronomic traits were observed. In the same combination of these uniform strains,there were strains segregating in the Mendelian manner. SSR markers analysis showed that F2 and F3 populations of the uniform strains and their F1 plants displayed the same markers indicating all the uniform strains were homozygous. All the marker bands of parents were simultaneously present on their progeny chromosomes, indicating that the uniform strains must true hybrid origin. The SSR markers of F2 population of segregating strains and their F1 plants showed that the segregating strains were heterzygosity. SSR marker band sites of RM276, RM258, RM248 and RM1 loci in most uniform strains presented at high frequencies bands site different from their parent's. We suggested that during the gamete formation,the genes that linked to ga-1, Rf and ga-8 mutated at certain frequency,and followed by a somatic meiosis of the fecundated zygote. Thus a haploid cell may develop into a homozygous embryo,and the F1 plant was homozygous and the progeny stabilized in early generation.

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